中文摘要：玉米赤霉烯酮（ZEN）由镰刀菌属产生，是一种引起牲畜繁殖障碍的雌激素类霉菌毒素。本研究描述了一种简单快速的方法，可以通过酯酶活性检测筛选出ZEN降解菌。经过试验，检测到一株名为短小芽孢杆菌ES-21的菌株,可以消除培养基中的ZEN。本研究提供了一种用于分离降解ZEN的微生物的策略以及一种有前景的降解菌株。
外文摘要：Zearalenone (ZEN), mainly produced by Fusarium species, is an estrogenic mycotoxin which causes reproductive disorders in livestock. In this study, we described a simple and rapid method for screening of ZEN-degrading bacteria by esterase activity assay. Soil bacteria strains were first tested for their esterase activities, then active strains were further evaluated for their ZEN-degrading potentials. A bacterial strain named Bacillus pumilus ES-21 was detected to be able to eliminate ZEN in the culture medium. ZEN degradation conditions were optimized through response surface methodology and the result showed that the degradation rate of ZEN by Bacillus pumilus ES-21 was up to 95.7% at the ZEN concentration of 17.9 g/ml within 24 h. One of the degradation product was proposed to be 1-(3,5-ihydroxypheny1)-6'-hydroxy-F-undecen-10'-one according to LC-TOF-MS/MS analysis. This study provided a strategy for the isolation of ZEN degrading microbes and a promising degrading strain.
外文关键词：Zearalenone；Biodegradation；Esterase activity；Bacillus pumilus
作者：Wang, G；Yu, MZ；Dong, F；Shi, JR；Xu, JH
作者单位：Jiangsu Acad Agr Sci
期刊名称：FOOD CONTROL
期刊影响因子：3.496
出版年份：2017
出版刊次：-
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