中文摘要：本研究的目的是评估来自单一孢子菌落的量化萌发、菌丝生长和黄曲霉毒素B1（AFB1）产生之间的时间关系，以发现能够在污染早期尽可能准确地预测AFB1出现的方法。本研究中，在次最优条件设定下，已经观测到萌发阶段离产生AFB1的时间较长。一旦生长率开始增加，需要六天时间来生产AFB1，当生长率到达100%后，只有40%-57%的AFB1生产检测被预测到。此外还发现，商品中菌落直径1-2mm可能是一个有用的AFB1污染和指标。
外文摘要：The aim of this work was to assess the temporal relationship among quantified germination, mycelial growth and aflatoxin B-1 (AFB1) production from colonies coming from single spores, in order to find the best way to predict as accurately as possible the presence of AFB1 at the early stages of contamination. Germination, mycelial growth, probability of growth and probability of AFB1 production of an isolate of Aspergillus flavus were determined at 25 degrees C and two water activities (0.85 and 0.87) on 3% Pistachio Extract Agar (PEA). The percentage of germinated spores versus time was fitted to the modified Gompertz equation for the estimation of the germination parameters (geometrical germination time and germination rate). The radial growth curve for each colony was fitted to a linear model for the estimation of the apparent lag time for growth and the growth rate, and besides the time to visible growth was estimated. Binary data obtained from growth and AFB1 studies were modeled using logistic regression analysis. Both water activities led to a similar fungal growth and AFB1 production. In this study, given the suboptimal set conditions, it has been observed that germination is a stage far from the AFB1 production process. Once the probability of growth started to increase it took 6 days to produce AFB1, and when probability of growth was 100%, only a 40-57% probability of detection of AFB1 production was predicted. Moreover, colony sizes with a radius of 1-2 mm could be a helpful indicator of the possible AFB1 contamination in the commodity. Despite growth models may overestimate the presence of AFB1, their use would be a helpful tool for producers and manufacturers; from our data 5% probability of AFB1 production (initiation of production) would occur when a minimum of 60% probability of growth is observed. Legal restrictions are quite severe for these toxins, thus their control from the early stages of contamination throughout the food chain is of paramount importance.
外文关键词：Germination；Growth；Single-spore；Aflatoxin probability；Predictive mycology
作者：Aldars-Garcia, L；Sanchis, V；Ramos, AJ；Marin, S
作者单位：Walailak Univ
期刊名称：FOOD MICROBIOLOGY
期刊影响因子：3.759
出版年份：2017
出版刊次：-
点击下载：一株黄曲霉在开心果琼脂上萌发、菌丝开始增殖并明显增长到产生可检测的AFB1的过程