中文摘要：轮状镰刀菌是一种全球性的玉米病菌，可能造成严重的粮食减产和经济损失，其有毒的次生代谢物还会威胁到人类和动物的健康。轮状镰刀菌和其它植物病原真菌含有大量未知基因，其中一些能够编码新型毒力因子或者抗真菌靶位。本研究利用功能基因鉴定并表征了轮状镰刀菌新型基因FUG1（真菌未知基因1）。分析结果认为FUG1代表了一种新型的真菌转录因子或者基因，不然就是参与了信号传导过程。
外文摘要：Fusarium verticillioides is a globally important pathogen of maize, capable of causing severe yield reductions and economic losses. In addition, F. verticillioides produces toxic secondary metabolites during kernel colonization that pose significant threats to human and animal health. Fusarium verticillioides and other plant-pathogenic fungi possess a large number of genes with no known or predicted function, some of which could encode novel virulence factors or antifungal targets. In this study, we identified and characterized the novel gene FUG1 (Fungal Unknown Gene 1) in F. verticillioides through functional genetics. Deletion of FUG1 impaired maize kernel colonization and fumonisin biosynthesis. In addition, deletion of FUG1 increased sensitivity to the antimicrobial compound 2-benzoxazolinone and to hydrogen peroxide, which indicates that FUG1 may play a role in mitigating stresses associated with host defence. Transcriptional profiling via RNA-sequencing (RNA-seq) identified numerous fungal genes that were differentially expressed in the kernel environment following the deletion of FUG1, including genes involved in secondary metabolism and mycelial development. Sequence analysis of the Fug1 protein provided evidence for nuclear localization, DNA binding and a domain of unknown function associated with previously characterized transcriptional regulators. This information, combined with the observed transcriptional reprogramming in the deletion mutant, suggests that FUG1 represents a novel class of fungal transcription factors or genes otherwise involved in signal transduction.
外文关键词：benzoxazinoids；DIMBOA；Fusarium ear rot；gene regulation；mycotoxin
作者：Ridenour, JB；Bluhm, BH
作者单位：Univ Arkansas
期刊名称：MOLECULAR PLANT PATHOLOGY
期刊影响因子：4.697
出版年份：2017
出版刊次：4
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