中文摘要：伏马菌素是一种有毒和致癌的霉菌毒素。本研究开发了一种基于胶体金标记的抗FB1的单克隆抗体探针的快速免疫色谱试纸，用于同时检测玉米中的伏马菌素 B1、B2和B3（FB1、FB2和FB3）。研究表明免疫色谱试纸可用于现场快速同时检测玉米样品中的FB1、FB2和FB3。
外文摘要：BACKGROUND Fumonisins are a kind of toxic and carcinogenic mycotoxin. A rapid immunochromatographic test strip has been developed for simultaneous detection of fumonisin B-1, B-2 and B-3 (FB1, FB2 and FB3) in maize based on colloidal gold-labelled monoclonal antibody(McAb) against FB1 probe.RESULTS The anti-FB1 McAb (2E11-H3) was produced through immunisation and cell fusion, and identified as high affinity, specificity and sensitivity. The cross-reaction ratios with fumonisin B-2 and B-3 were accordingly 385% and 72.4%, while none with other analogues. The colloid gold-labelled anti-FB1 McAb probe was successfully prepared and used for establishing the immunochromatographic strip. The test strip showed high sensitivity and specificity, the IC50 for FB1 was 58.08 ng mL(-1), LOD was 11.24 ng mL(-1), calculated from standard curve. Moreover, the test strip exhibited high cross-reactivity with FB2 and FB3, and could be applied to the simultaneous detection of FBs (FB1:FB2:FB3 = 12:4:1) in maize sample with high accuracy and precision. The average recoveries of FBs in maize ranged from 90.42% to 95.29%, and CVs were 1.25-3.77%. The results of the test strip for FBs samples showed good correlation with high-performance liquid chromatography analysis.Conclusion The immunochromatographic test strip could be employed in the rapid simultaneous detection of FB1, FB2 and FB3 in maize samples on-site.
外文关键词：fumonisins；monoclonal antibody；colloidal gold；Immunochromatographic test strip
作者：Yao, JJ；Sun, YN；Li, QM； Wang,FY；Teng, M；Yang, YY；Deng, RG；Hu, XF
作者单位：Henan Acad Agr Sci
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.076
出版年份：2017
出版刊次：7
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