中文摘要：必需微量元素硒（Se）的循环浓度在炎症性疾病中显著降低，虽然硒主要通过25种硒蛋白的功能发挥其生理作用，但硒蛋白基因在免疫组织炎症反应中的反应仍不清楚。本研究旨在确定在脂多糖（LPS）诱导的猪炎症反应中免疫组织中Se的保留和硒蛋白基因的表达。研究发现LPS诱导的炎症反应会使Se代谢受损，并且与免疫组织中硒蛋白基因表达的失调相关。
外文摘要：Circulating concentration of the essential trace element selenium (Se) was significantly lower in inflammatory disorders. Although Se plays physiological roles mainly through the function of 25 selenoproteins, the response of the selenogenome in immune tissues during inflammatory reactions remains unclear. The objective of this study was to determine the Se retention and selenogenome expression in immune tissues during the lipopolysaccharide (LPS)-induced inflammatory response in porcine. A total of 12 male pigs were randomly divided into two groups and injected with LPS or saline. After 4 h postinjection, blood samples were collected and pigs were euthanized. Pigs challenged with LPS had 36.8 and 16.6 % lower (P < 0.05) Se concentrations in the serum and spleen,respectively, than those injected with saline. Moreover, the activities of GPX decreased (P < 0.05) by 23.4, 26.6, and 30.4 % in the serum,thymus, and lymph node, respectively, in the pigs injected with LPS.Furthermore, the LPS challenge altered (P < 0.05) the mRNA expression of 14, 16, 10, and 6 selenoprotein genes in the liver, spleen, thymus, and lymph node, respectively. Along with 10 previously reported selenoprotein genes, the response of Txnrd2, Txnrd3, Sep15, Selh, Seli, Seln, Selo, Selt, Selx, and Sephs2 to inflammatory reaction in immune tissues were newly illustrated in this study. In conclusion, the LPS-induced inflammatory response impaired Se metabolism and was associated with dysregulation of the selenogenome expression in immune tissues.
 
外文关键词：elenium；Selenoprotein；LPS； Inflammatory reaction；Pigs
作者：Sun, LH；Pi, DA；Zhao, L；Wang, XY；Zhu, LY；Qi, DS；Liu, YL
作者单位：Huazhong Agr Univ
期刊名称：BIOLOGICAL TRACE ELEMENT RESEARCH
期刊影响因子：1.789
出版年份：2017
出版刊次：1
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