中文摘要：环AMP信号控制生物体对细胞外刺激做出反应的一系列生理过程。在信号级联中，作为第二信使的cAMP由腺苷酸环化酶（生物合成）和CAMP磷酸二酯酶（PDE）（水解）控制。在本研究中，我们探讨了产生致癌物黄曲霉毒素的真菌黄曲霉的高亲和力（PdeH）和低亲和力（PdeL）cAMP磷酸二酯酶的功能，并发现与PdeL不同的是，PdeH的失活表现出分生孢子和核仁形成的减少。研究结果表明，PdeH在黄曲霉的营养生长发育和黄曲霉毒素生物合成中起主要作用，但与PdeL具有重叠作用。
外文摘要：Cyclic AMP signaling controls a range of physiological processes in response to extracellular stimuli in organisms. Among the signaling cascades, cAMP, as a second messenger, is orchestrated by adenylate cyclase (biosynthesis) and CAMP phosphodiesterases (PDEs) (hydrolysis). In this study, we investigated the function of the high-affinity (PdeH) and low-affinity (PdeL) cAMP phosphodiesterase from the carcinogenic aflatoxin producing fungus Aspergillus flavus, and found that instead of PdeL, inactivation of PdeH exhibited a reduction in conidiation and sclerotia formation. However, the Delta pdeL/Delta pdeH mutant exhibited an enhanced phenotype defects, a similar phenotype defects to wild-type strain treated with exogenous CAMP. The activation of PICA activity was inhibited in the Delta pdeH or Delta pdeL/Delta pdeH mutant, both of whom exhibited increasing AF production. Further analysis by qRT-PCR revealed that pdeH had a high transcriptional level compared to pdeL in wild-type strain, and affected pdeL transcription. Green fluorescent protein tagging at the C-terminus of PDEs showed that PdeH-GFP is broadly compartmentalized in the cytosol, while PdeL-GFP localized mainly to the nucleus. Overall, our results indicated that PdeH plays a major role, but has overlapping function with PdeL, in vegetative growth, development and AF biosynthesis in A. flavus.
外文关键词：Phosphodiesterase, Sclerotia, cAMP, PKA
作者：Yang,KL；Liu,YH；Liang,LL；Li,ZG；Qin,QP；Nie,XY；Wang,SH
作者单位：Fujian Agr & Forestry Univ
期刊名称：FUNGAL GENETICS AND BIOLOGY
期刊影响因子：2.933
出版年份：2017
出版刊次：101
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