中文摘要：本研究对肉鸡及猪静脉注射和口服给药脱氧雪腐镰刀菌烯醇-3-β-d-葡糖苷(DON3G)和脱氧雪腐镰刀菌烯醇(DON)，进行交叉动物试验。结果发现虽然体外研究表明DON3G比DON的毒性低，但依赖于物种的毒代动力学数据及其在体内水解生成DON，说明了毒理学相关性，因此需要进一步研究，以建立每日耐受量。
外文摘要：Crossover animal trials were performed with intravenous and oral administration of deoxynivalenol-3-β-d-glucoside (DON3G) and deoxynivalenol (DON) to broiler chickens and pigs. Systemic plasma concentrations of DON, DON3G and de-epoxy-DON were quantified using liquid chromatography–tandem mass spectrometry. Liquid chromatography coupled to high-resolution mass spectrometry was used to unravel phase II metabolism of DON. Additionally for pigs, portal plasma was analysed to study presystemic hydrolysis and metabolism. Data were processed via tailor-made compartmental toxicokinetic models. The results in broiler chickens indicate that DON3G is not hydrolysed to DON in vivo. Furthermore, the absolute oral bioavailability of DON3G in broiler chickens was low (3.79 ± 2.68 %) and comparable to that of DON (5.56 ± 2.05 %). After PO DON3G administration to pigs, only DON was detected in plasma, indicating a complete presystemic hydrolysis of the absorbed fraction of DON3G. However, the absorbed fraction of DON3G, recovered as DON, was approximately 5 times lower than after PO DON administration, 16.1 ± 5.4 compared with 81.3 ± 17.4 %. Analysis of phase II metabolites revealed that biotransformation of DON and DON3G in pigs mainly consists of glucuronidation, whereas in chickens predominantly conjugation with sulphate occurred. The extent of phase II metabolism is notably higher for chickens than for pigs, which might explain the differences in sensitivity of these species to DON. Although in vitro studies demonstrate a decreased toxicity of DON3G compared with DON, the species-dependent toxicokinetic data and in vivo hydrolysis to DON illustrate the toxicological relevance and consequently the need for further research to establish a tolerable daily intake.
外文关键词：Modified mycotoxins；Masked mycotoxins；Fusarium toxins；Animal trials
作者：Broekaert, N；Devreese, M；van Bergen, T；Schauvliege, S；De Boevre, M；De Saeger, S；Vanhaecke, L；Berthiller, F；Michlmayr, H；Malachova, A；Adam, G；Vermeulen, A；Croubels, S
作者单位：Univ Ghent
期刊名称：ARCHIVES OF TOXICOLOGY
期刊影响因子：6.637
出版年份：2017
出版刊次：2
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