中文摘要：表达Cry基因（来自苏云金芽胞杆菌Bt）的转基因水稻对鳞翅目害虫具有高抗性。褐飞虱是转Bt基因水稻的主要非靶标吮吸式害虫。池塘狼蛛是稻田中褐飞虱最主要的天地。因此，转Bt基因水稻对褐飞虱和池塘狼蛛的安全评价应在其商业化之前开展。本文进行了两个实验来评估转Bt基因水稻对褐飞虱和池塘狼蛛的潜在生态影响：（1）进行营养实验来评估不同的Cry1Ac、Cry2Aa和Cry1Ca蛋白在食物链中的传输；和（2）综合分析cry2Aa和Cry1Ca对褐飞虱和池塘狼蛛的回肠绒毛刷状缘膜蛋白抗性。研究发现，褐飞虱取食的转Bt基因水稻只有微量的Cry1Ac、Cry2Aa或Cry1Ca蛋白，且只有Cry1Ac和Cry2Aa蛋白可以通过捕食从褐飞虱传输到池塘狼蛛。因此，我们推断，转Bt基因水稻品种对褐飞虱和池塘狼蛛的影响可以忽略不计。
外文摘要：Transgenic rice expressing cry genes from the bacterium Bacillus thuringiensis (Bt rice) is highly resistant to lepidopteran pests. The brown planthopper (BPH, Nilaparvata lugens) is the main non-target sapsucking insect pest of Bt transgenic rice. The pond wolf spider (PWS, Pardosa pseudoannulata) is one of the most dominant predators of BPH in rice fields. Consequently, the safety evaluation of Bt rice on BPH and PWS should be conducted before commercialization. In the current study, two experiments were performed to assess the potential ecological effects of Bt rice on BPH and PWS: (1) a tritrophic experiment to evaluate the transmission of Cry1Ac, Cry2Aa and Cry1Ca protein in the food chain; and (2) binding assays of Cry1Ac, Cry2Aa and Cry1Ca to midgut brush border membrane proteins from BPH and PWS. Trace amounts of the three Cry proteins were detected in BPH feeding on Bt rice cultivars, but only Cry1Ac and Cry2Aa proteins could be transferred to PWS through feeding on BPH. In vitro binding of biotinylated Cry proteins and competition assays in midgut protein vesicles showed weak binding, and ligand blot analysis confirmed the binding specificity. Thus, we inferred that the tested Bt rice varieties have negligible effects on BPH and PWS.
作者：Niu, Lin; Mannakkara, Amani; Qiu, Lin; 等
作者单位：Huazhong Agr Univ
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.259
出版年份：2017
出版刊次：5
点击下载：转Bt基因水稻品系产生的Cry1Ac、Cry2Aa或Cry1Ca蛋白对褐飞虱和池塘狼蛛没有不利影响