中文摘要：玉米赤霉烯酮（ZEA）是一种人类食品和动物饲料的污染物，能引发健康危害。但是，玉米赤霉烯酮的毒性信号通路尚未阐明。本研究旨在弄清哪种信号通路参与了ZEA诱导的细胞凋亡并研究在ZEA处理期，永生化山羊睾丸间质细胞系hTERT-GLC中凋亡诱导因子（AIF）对细胞凋亡的影响。研究发现ZEA通过不同的信号通路诱导hTERT-GLCs细胞凋亡，而AIF介导的信号通路在ZEA诱导的hTERT-GLCs细胞凋亡中起着关键作用。
外文摘要：Zearalenone (ZEA) is a contaminant of human food and animal feedstuffs that causes health hazards. However, the signal pathways underlying ZEA toxicity remain elusive. The aims of this study were to determine which pathways are involved in ZEA-induced cell death and investigate the effect of apoptosis inducing factor (AIF) on cell death during ZEA treatment in the immortalized goat Leydig cell line hTERT-GLC. This study showed that ZEA-induced cell death in hTERT-GLCs works via endoplasmic reticulum (ER) stress, the caspase-dependent pathway, the caspase-independent pathway and autophagy. Recombinant lentiviral vectors were constructed to silence AIF expression in hTERT-GLCs. Flow cytometry results showed that knockdown of AIF diminished ZEA-induced cell apoptosis in hTERT-GLCs. Furthermore, we found AIF depletion down-regulated phosphoIRE1α, GRP78, CHOP and promoted the switch of LC3-I to LC3-II. Therefore, ZEA induces cytotoxicity in hTERT-GLCs via different pathways, while AIF-mediated signaling plays a critical role in ZEA-induced cell death in hTERT-GLCs.
外文关键词：Zearalenone；Goat；Apoptosis inducing factor；Endoplasmic reticulum stress
作者：Yang, DQ；Jiang, TT；Lin, PF；Chen, HT；Wang, L；Wang, N；Zhao, F；Tang, KQ；Zhou, D；Wang, AH；Jin, YP
作者单位：Northwest A&F Univ
期刊名称：REPRODUCTIVE TOXICOLOGY
期刊影响因子：2.85
出版年份：2017
出版刊次：1
点击下载：在山羊睾丸间质细胞系中凋亡诱导因子基因缺失抑制玉米赤霉烯酮诱导的细胞凋亡