中文摘要：免疫测定法因其快速、选择性和灵敏的特点，成为广泛采用的检测黄曲霉毒素的技术。但他们共同的缺点是使用黄曲霉毒素作为标准物质，可能因处理不当引起对操作人员的暴露风险和环境污染。抗独特型抗体（anti-Ids或Ab2s），又叫内影像型抗独特型抗体，能够模拟并充当抗原，因此，使用抗独特型抗体作为替代品，开发并验证了黄曲霉毒素B1(AFB1)的无毒酶联免疫吸附测定（ELISA）方法。研究表明抗独特型抗体可以模拟AFB1内影像，它可以在免疫测定中替代抗原，降低操作者的操作风险和环境污染。
外文摘要：BACKGROUND:Immunoassays are widely employed techniques to detect aflatoxins since they are rapid, selective and sensitive. One common disadvantage of them is using aflatoxins as standard substances, which may trigger exposure risks to operators and environmental contamination without proper handling. Anti-idiotypic antibodies (anti-Ids or Ab2s), also named as internal-image anti-Ids, are able to mimic and function as antigens, so a non-toxic enzyme-linked immunosorbent assay (ELISA) for aflatoxin B1 (AFB1 ) is developed and validated using anti-Ids as substitutes.
RESULTS:Mouse monoclonal anti-idiotypic antibody (McAb2) to AFB1 was generated by the hybridoma technique using Fab fragments of rabbit anti-AFB1 idiotype antibody (Ab1) as immunogen. As indicated by indirect competitive ELISA, McAb2, represented an internal-image of antigen AFB1 , was able to bind Fab with competition to AFB1 . Then, analysis of AFB1 in spiked samples by non-toxic ELISA using anti-Ids as substitutes was developed, and it showed no significant differences with comparison to AFB1 as competitive antigens.
CONCLUSION:Our work demonstrated that anti-Ids could be used as internal-image mimicry of AFB1 , and it had potential applications in immunoassays for antigen substitution to reduce operational risk for operators and environmental contamination.
外文关键词：aflatoxin B1；anti-idiotypic antibody；Fab fragment；internal-image
作者：Hu, L；Liu, AP；Chen, WF；Yang, HX；Wang, XH；Chen, FS
作者单位： Huazhong Agr Univ
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.076
出版年份：2017
出版刊次：5
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