从葡萄及葡萄干中分离的炭黑曲霉在赭曲霉毒素A生物合成过程中挥发性有机化合物的积累及其相互关系的研究

A study on accumulation of volatile organic compounds during ochratoxin a biosynthesis and characterization of the correlation in Aspergillus carbonarius isolated from grape and dried vine fruit

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中文摘要:在炭黑曲霉菌株(AF, SD26和SD27) 7天的生长期中分别用HS-SPME-GC-MS和HPLC-MS/MS检测到了挥发性有机化合物(VOCs)和赭曲霉毒素A(OTA)。该分析表明C-8醇、酮及橙花叔醇与OTA的合成有密切的关系。
外文摘要:Volatile organic compounds (VOCs) and ochratoxin A (OTA) were detected in Aspergillus carbonarius strains (AF, SD26 and SD27) during a 7-day growing period, using HS-SPME-GC-MS and HPLC-MS/MS, respectively. Results showed that 33 VOCs were detected in these strains. The AF strain showed the different sesquiterpene profile compared with the other strains. Principal component analysis revealed that the AF strain was segregated from the other two strains regarding the VOC profile, especially the sesquiterpene profile. Ester metabolites were produced more at the beginning of the strain growth period, whereas extension of the strain incubation resulted in an intense accumulation of hydrocarbon compounds. The prediction models on the correlation between OTA and VOCs were established using partial least squares regression analysis. This analysis indicated that C-8 alcohols, ketones, and trans-nerolidol showed a close relation with the OTA synthesis.
外文关键词:Ochratoxin A;Aspergillus carbonarius;Volatile organic compounds;PCA;PLSR model
作者:Zhang, XX;Cheng, Z;Ma, LY;Li, JM
作者单位:China Agr Univ
期刊名称:FOOD CHEMISTRY
期刊影响因子:4.052
出版年份:2017
出版刊次:6
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  1. 编译服务:农产品质量安全
  2. 编译者:虞德容
  3. 编译时间:2017-08-07