中文摘要：本研究成功开发了一种简易的检测玉米赤霉烯酮（ZEN）的方法。研究表明该新型的适配体传感器用于快速、准确地检测ZEN，潜力巨大。
外文摘要：A facile strategy was successfully developed for the detection of zearalenone (ZEN). In this assay, highly fluorescent upconversion nanoparticles were synthesized and conjugated with the complementary oligonucleotide of ZEN aptamer for use as signal probes. Magnetic nanoparticles immobilized with the ZEN aptamer were assigned as capture probes. The results exhibited that the linear correlation between the decreased luminescence intensity of the signal and the concentration of ZEN was very strong (R2 = 0.9957) in the range of 0.05–100 μg/L. In addition, the limit of detection of the proposed method (0.126 μg/kg for corn and 0.007 μg/L for beer) was significantly lower than the existing methods. Furthermore, the reliability of the competitive immunoassay format was validated by comparing the results determined in real food samples to those obtained from a commercially available method. Overall, the novel aptasensor have showed great potential for rapid and accurate ZEN determination.
外文关键词：Biosensor；Zearalenone；Mycotoxin；Fluorescence；Magnetic nanoparticles
作者：Wu, ZZ；Xu, EB；Chughtai, MFJ；Jin, ZY；Irudayaraj, J
作者单位：Jiangnan Univ
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：9
点击下载：用新型基于上转换纳米粒子的适配体传感器高度灵敏地荧光检测玉米赤霉烯酮