中文摘要：黄曲霉毒素B1（AFB1）污染食品和饲料已造成全球严重的经济和健康问题，迫切需要开发出黄曲霉毒素降解的生物学方法。研究发现镰刀菌WCQ3361是第一个报道的能降解AFB1的镰刀菌属真菌，有着宽泛的反应温度范围、优良的热稳定性及高活性，为处理人类膳食及动物饲料中的AFB1提供了一个潜在的非常有用的解决方案。
外文摘要：BACKGROUND: Contamination of food and feed by Aflatoxin B1 (AFB1) has posed serious economic and health problem worldwide, developing biological methods for AFB1 degradation is highly required. RESULTS: Among three AFB1 degrading microorganisms isolated from moldy peanut, Fusarium sp. WCQ3361 could remove AFB1 extremely effective with a degradation ratio of 70.20% after 1 min and 95.38% after 24 h. Its degradation ratio was not much affected by temperature change (0-90 °C), and it also displayed excellent thermostability, maintained 99.40% residual activity after boiling for 10 min. Since protease K could reduce the AFB1 degradation ratio by 55.15 %, we proposed that the effective component for AFB1 degradation is a protein. The AFB1 degradation ability of Fusarium sp. WCQ3361 was further verified by feed stock detoxication and MTT test with HepG2 cells. In addition, no degradation products were detected by preliminary liquid chromatography mass spectrometry, suggesting AFB1 might be metabolized to products with different chemical characteristics from AFB1. CONCLUSION: Fusarium sp. WCQ3361 was the first reported AFB1 degradation fungus belonging to genus Fusarium with broad reaction temperature range, excellent thermostability and high activity, which provides a potential highly useful solution to dealing with AFB1 contamination in human diet and animal feed.
外文关键词：aflatoxin B1；biodegradation；Fusarium sp；broad working temperature；thermostability；MTT assay；liquid chromatography/mass spectrometry
作者：Wang, CQ；Li, ZY；Wang, H；Qiu, HY；Zhang, MH；Li, S；Luo, XG；Song, YJ；Zhou, H；Ma, WJ；Zhang, TC
作者单位：Tianjin Univ Sci & Technol
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.076
出版年份：2017
出版刊次：4
点击下载：用反应温度范围宽泛且热稳定性优良的镰刀菌WCQ3361的代谢物快速生物降解黄曲霉毒素B1