中文摘要：本研究开发了一种电感耦合等离子体质谱(ICP-MS)免疫分析法用于检测牛奶样品中超痕量水平的黄曲霉毒素M1(AFM1)。研究表明该方法比文献报道的其它免疫法动态工作范围(0.012–2.5μg/kg)更高。
外文摘要：A n inductively coupled plasma mass spectrometry (ICP-MS)-based immunoassay has been developed to quantify aflatoxin M1 (AFM1) at ultra-trace levels in milk samples. AFM1 detection is carried out by means of a competitive immunoassay using secondary biotinylated antibodies and streptavidin-conjugated Au nanoparticles. After acid addition, nanoparticles are decomposed and Au signal is registered by means of ICP-MS. Results demonstrate that, under optimum conditions, the limit of detection of the immunoassay (0.005 μg kg−1) is low enough to quantify AFM1 according to current international policies (including the more restrictive European one). Method accuracy and precision was checked by analyzing an AFM1 certified reference material and different milk samples spiked with known amounts of AFM1. AFM1 recovery values range from 80% to 102% whereas inter-assay and intra-assay precision are lower than 15%. Finally, this immunoassay methodology affords a higher dynamic working range (0.012–2.5 μg kg−1) than other immunoassay methodologies described in the literature.
外文关键词：Aflatoxin M1；Milk；Immunoassay；Gold nanoparticles；Inductively coupled plasma mass spectrometry
作者：Perez, E；Martinez-Peinado, P；Marco, F；Gras, L；Sempere, JM；Mora, J；Grindlay, G
作者单位：Univ Alicante
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：9
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