中文摘要：豇豆 (Vigna unguiculata)是一种营养丰富的豆科作物，在全球发展中国家有许多农户种植。已知镰刀菌及伏马菌素B1（FB1）与豇豆种子相关。开展本研究旨在确定FB1的植物毒性对豇豆苗的影响并为研究毒素对神经酰胺合成酶基因表达的影响提供思路。研究发现FB1不影响豇豆神经酰胺合成酶基因的表达。虽然FB1对生长的豇豆苗表现出植物毒性效应，但观察到的生理和形态变化却并非神经酰胺合成酶基因表达下调的结果。
外文摘要：Cowpea (Vigna unguiculata) is a nutritious leguminous crop cultivated by many smallholder farmers in developing countries of the world. Fusarium spp. and fumonisin B1 (FB1) are known to be associated with cowpea seed. This study was performed to determine the phytotoxic effects of FB1 on cowpea seedlings and to provide insight on the effect of the toxin on ceramide synthase gene expression. Surface-disinfected seeds were imbibed in FB1 solutions of 5, 20 and 40 mg/L concentrations. Additionally, seeds were artificially inoculated with three different FB1-producing Fusarium verticillioides strains. Percentage emergence was reduced by all three FB1 concentrations and seeds inoculated with F. verticillioides strain MRC 8265. Seedlings imbibed in 40 mg/L FB1 had reduced seedling length whilst seedling mass was reduced by all treatments. Total chlorophyll content was higher in leaves of seedlings raised from seeds imbibed in all three FB1 concentrations when compared to the controls. To evaluate the effect of FB1 on ceramide synthase gene expression, seeds were imbibed in a 20 mg/L FB1 solution. RNA was extracted from untreated and treated samples after 0, 3 and 12 dpi with the latter being divided into shoot and roots samples. Semi-quantitative reverse transcription PCR suggested that there were no significant differences in ceramide synthase gene expression between the control and toxin treated cowpea samples. It thus appears that FB1 did not influence ceramide synthase gene expression in cowpea. Although FB1 exhibited phytotoxic effects on developing cowpea seedlings, the observed physiological and morphological changes were not a result of down regulation of ceramide synthase gene expression.
外文关键词：Phytotoxicity；Fumonisin B-1；Seedling vigour index (SVI)；Vigna unguiculata；Ceramide synthase；Gene expression
作者：Kotze, RG；Crampton, BG；Kritzinger, Q
作者单位：Univ Pretoria
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY
期刊影响因子：1.495
出版年份：2017
出版刊次：2
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