中文摘要：本研究旨在用聚苯乙烯-二乙烯基苯(PS-DVB)作柱填料创建一种同时检测四种镰刀菌毒素（ZEN, DON、T-2及HT-2毒素）的免疫亲和净化柱（IAC）。本研究开发了一种新的用PS-DVB树脂作柱填料制备多目标IAC检测食品中镰刀菌毒素的方法，并将GALAK IAC用于面粉样品检测其在实际样品中应用的可行性。
外文摘要：The aim of this study was to establish a purifying immunoaffinity column (IAC) for four kinds of Fusarium toxins (ZEN, DON, T-2, and HT-2 toxins) simultaneously, by using polystyrene-divinylbenzene (PS-DVB) as a column matrix. Four different types of PS-DVB resins were selected to prepare the IACs. Among them, two types of resins, GALAK and Agela, were chosen for IAC preparation. The coupling ratios of the monoclonal antibodies and GALAK or Agela resins were 98.37 and 97.64%, the recoveries of the four Fusarium toxins on the GALAK or Agela IACs were 73.02∼91.97 or 66.16∼112.80%, and the RSDs of recoveries were 2.17∼16.53 or 3.42∼12.25%. They can be applied for the purification of Fusarium toxins from the actual food samples. The GALAK resin was a type of matrix with hydrophilic treatment. The recoveries of four Fusarium toxins on Agela IAC were either too low or too high. The adsorption capacities of the GALAK IAC for the four Fusarium toxins were better than those of the Agela IAC. Hence, the research developed a new method for the preparation of multi-target IAC using PS-DVB resin as matrix of column for determination of Fusarium toxins in food. Then, the GALAK IAC was used for flour samples to detect the feasibility of the application in the actual sample.
外文关键词：Polystyrene-divinylbenzene；Immunoaffinity columns；Fusarium toxins
作者：Zhang, JN；Ding, K；Han, T；Chen, XN
作者单位：Beijing Agr Univ
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2017
出版刊次：6
点击下载：用聚苯乙烯-二乙烯基苯为柱填料制备多目标镰刀菌毒素（玉米赤霉烯酮、脱氧雪腐镰刀菌毒素、T-2及HT-2毒素）免疫亲和柱