中文摘要：本文用三重四极杆在发生研究中优化、验证并应用了一种快速单次萃取法用于超高效液相色谱-串联质谱同时检测玉米中黄曲霉毒素B1、B2、G1、G2、伏马菌素B1及玉米赤霉烯酮。研究表明该方法在环境影响、效率和可靠性方面表现出了相当的优势。
外文摘要：A single-extraction method to simultaneously determine aflatoxins (B1, B2, G1, G2), fumonisin B1, and zearalenone in corn meal by ultra performance liquid chromatography tandem mass spectrometry, using a triple quadrupole, was optimized, validated, and applied in an occurrence study. Different extraction solutions were tested, with better performance for methanol/acetonitrile/water (60:20:20, v/v/v). Linearity was observed from 0.25 to 1.50 ng/mL for aflatoxins, from 20 to 120 ng/mL for fumonisin, and from 7.00 to 42.00 ng/mL for zearalenone. Significant matrix effects were shown for all groups. Selectivity was demonstrated, as matrix or spectral interferences were not observed at the predicted retention time window of the target analytes. Average recoveries of 87.57, 93.18, 93.35, 94.20, 78.76, and 95.98% were obtained for aflatoxins (B1, B2, G1, and G2) fumonisin and zearalenone, respectively. A z-score of 0.19 was estimated in a corn certified reference material for fumonisin B1. Maximum relative standard deviation values under repeatability and intermediate precision conditions were determined to be 13.6 and 13.6% for aflatoxins, 3.7 and 6.3% for fumonisin, and 3.5 and 4.0% for zearalenone, respectively. In the occurrence study, 50 samples were analyzed and 44% had measurable levels of fumonisin. Zearalenone was detected in 18%. The proposed method showed considerable advantages, considering environmental impacts, efficiency, and reliability.
外文关键词：Mycotoxins；Food；Mass spectrometry；Multigroup determination；Single-laboratory validation；Application
作者：Paschoal, FN；Silva, DD；de Souza, RV；de Oliveira, MS；Pereira, DAA；de Souza, SVC
作者单位：Fed Univ Minas Gerais UFMG
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2017
出版刊次：6
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