中文摘要：多胺参与一些重要的细胞功能，因而对所有生物体都非常重要。多胺的生物合成途径及其调节已被公认，并且在此意义上，鸟氨酸辅羧酶（ODC）是一种控制整个通路的要素。本研究评估了烟草赤星病菌的二氟甲基鸟氨酸（DFMO）对ODC的抑制作用，并发现其减少了真菌生长及霉菌毒素的产生。随着抗药性问题的出现，寻找新型杀菌剂已成为人们的兴趣所在。
外文摘要：Polyamines are essential for all living organisms as they are involved in several vital cell functions. The biosynthetic pathway of polyamines and its regulation is well established and, in this sense, the ornithine descarboxylase (ODC) enzyme acts as one of the controlling factors of the entire pathway. In this work we assessed the inhibition of the ODC with D, l-α-difluoromethylornithine (DFMO) on Alternaria alternata and we observed that fungal growth and mycotoxin production were reduced. This inhibition was not completely restored by the addition of exogenous putrescine. Actually, increasing concentrations of putrescine on the growth media negatively affected mycotoxin production, which was corroborated by the downregulation of pksJ and altR, both genes involved in mycotoxin biosynthesis. We also studied the polyamine metabolism of A. alternata with the goal of finding new targets that compromise its growth and its mycotoxin production capacity. In this sense, we tested two different polyamine analogs, AMXT-2455 and AMXT-3016, and we observed that they partially controlled A. alternata’s viability in vitro and in vivo using tomato plants. Finding strategies to design new fungicide substances is becoming a matter of interest as resistance problems are emerging.
外文关键词：Alternaria alternata；Alternariol；Alternariol monomethyl ether；Polyamines；DFMO Polyamine transport inhibitor
作者：Estiarte, N；Crespo-Sempere, A；Marin, S；Sanchis, V；Ramos, AJ
作者单位：Univ Lleida
期刊名称：FOOD MICROBIOLOGY
期刊影响因子：3.682
出版年份：2017
出版刊次：8
点击下载：研究烟草赤星病菌多胺代谢，寻求控制真菌感染的新物质