中文摘要：本研究旨在开发一种高灵敏度的免疫层析法检测植物提取物中的伏马菌素B1。该方法灵活性高，可用于检测范围广泛的低分子化合物。免疫层析中使用抗物种抗体结合金纳米粒子，无需合成并表征用特异性抗体为每一种新的待测物形成的结合物，从而显著促进了检测系统的发展。
外文摘要：To develop a high-sensitivity immunochromatographic test for fumonisin B1 in plant extracts. Unlike conventional immunochromatographic tests, this assay is performed in two stages: competitive reaction with free specific antibodies and identifying immune complexes by their interaction with the anti-species antibody-conjugated gold nanoparticles. The use of a new geometry for the test strip membranes and a novel reagent application method ensures the proper order of these stages without additional manipulations. The contact of the ready-to-use test strip with the liquid sample suffices in initiating all stages of the assay and obtaining test results. The developed test was used on corn extracts; its instrumental limit of fumonisin B1 detection was 0.6 ng ml-1 at 15 min of assay duration. The proposed approach is flexible and can be used for a wide range of low molecular compounds. The use of anti-species antibody-conjugated gold nanoparticles in immunochromatography significantly facilitates the development of test systems by eliminating the need to synthesize and characterize the conjugates with specific antibodies for each new compound to be detected.
外文关键词：Fumonisin B1；Gold nanoparticles；Immunochromatography；Indirect labeling；Sensitivity enhancement；Test strip
作者：Urusov, AE；Petrakova, AV；Gubaydullina, MK；Zherdev, AV；Eremin, SA；Kong, DZ；Liu, LQ；Xu, CL；Dzantiev, BB
作者单位：Russian Acad Sci
期刊名称：BIOTECHNOLOGY LETTERS
期刊影响因子：1.639
出版年份：2017
出版刊次：5
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