中文摘要：在丝状真菌中发现了不同数量的质子耦合双肽转运体，PTR2s，但它们在真菌发育及植物侵染中的作用尚不明确。本研究从引起谷物赤霉病的禾谷镰刀菌中，发现了四种PTR2转运体(FgPTR2A-D)。已知性发育及次生代谢物的产生与调节水平相关，而本研究结果显示PTR2s在氮转化中很活跃，从而影响了信号进程。
外文摘要：Putative proton coupled di-peptide transporters, PTR2s, are found in filamentous fungi in different numbers and their function during fungal development and plant infection is unresolved. In Fusarium graminearum, the cause of head blight in cereals, we identified four putative PTR2 transporters (FgPTR2A-D). The genes did not cluster together in phylogenetic analyses and only FgPTR2A and FgPTR2C were able to complement a PTR2 deficient yeast mutant in uptake of di-peptides. All FgPTR2s are continuously expressed throughout the fungal lifecycle, although at different levels. In silico analyses of existing expression-data show that FgPTR2B is found at higher levels than the others in planta and during sexual development. Deletion mutants of FgPTR2A, FgPTR2C, and FgPTR2D had a higher production of deoxynivalenol (DON) and zearalenone and lower production of fusarielin H than the wild type. Perithecium development was reduced in these mutants but unaffected by deletion of FgPTR2B. Conidia production was reduced in the FgPTR2B mutant and unaffected by deletion of the other PTR2 transporters. Sexual development and secondary metabolite production are known to be linked at the regulatory level and the results suggest that PTR2s are active in nitrogen turnover and thereby influence signal processes.
外文关键词：Di/tri peptide transporters；Fungal development；Peptide uptake；Regulation；Secondary metabolites
作者：Droce, A；Sorensen, JL；Sondergaard, TE；Rasmussen, JJ；Lysoe, E；Giese, H
作者单位：Aalborg Univ
期刊名称：FUNGAL BIOLOGY
期刊影响因子：2.244
出版年份：2017
出版刊次：5
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