中文摘要：发达国家越来越多的人面临着大肠杆菌的威胁，它能产生一种被怀疑与大肠癌发展有关的遗传毒素，大肠杆菌素。脱氧雪腐镰刀菌烯醇（DON）在欧洲和北美是污染主食，特别是谷物制品的最普遍的霉菌毒素。本研究调查了食品污染物DON对产毒大肠杆菌遗传毒性的作用。结果表明产毒大肠杆菌的遗传毒性，越来越多地出现在无症状的人的微生物群中，受膳食中存在的DON的调节。这就提出了关于食品污染物和肠道菌群对肠道癌变的协同作用的问题。
外文摘要：An increasing number of human beings from developed countries are colonized by Escherichia coli strains producing colibactin, a genotoxin suspected to be associated with the development of colorectal cancers. Deoxynivalenol (DON) is the most prevalent mycotoxin that contaminates staple food-especially cereal products-in Europe and North America. This study investigates the effect of the food contaminant DON on the genotoxicity of the E. coli strains producing colibactin. In vitro, intestinal epithelial cells were coexposed to DON and E. coli producing colibactin. In vivo, newborn rats colonized at birth with E. coli producing colibactin were fed a DON-contaminated diet. Intestinal DNA damage was estimated by the phosphorylation of histone H2AX. DON exacerbates the genotoxicity of the E. coli producing colibactin in a time- and dose-dependent manner in vitro Although DON had no effect on the composition of the gut microbiota, and especially on the number of E. coli, a significant increase in DNA damage was observed in intestinal epithelial cells of animals colonized by E. coli strains producing colibactin and coexposed to DON compared to animals colonized with E. coli strains unable to produce colibactin or animals exposed only to DON. In
conclusion, our data demonstrate that the genotoxicity of E. coli strains producing colibactin, increasingly present in the microbiota of asymptomatic human beings, is modulated by the presence of DON in the diet. This raises questions about the synergism between food contaminants and gut microbiota with regard to intestinal carcinogenesis.
作者：Payros, D；Dobrindt, U；Martin, P；Secher, T；Bracarense, APFL；Boury, M；Laffitte, J；Pinton, P；Oswald, E；Oswald, IP
作者单位：Univ Toulouse
期刊名称：MBIO
期刊影响因子：6.975
出版年份：2017
出版刊次：2
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