中文摘要：本研究旨在评估硒代蛋氨酸（SeMet）对AFB1诱导的鸡原代肝细胞损伤的保护作用。研究表明，SeMet可以通过增强SelW的表达来抑制CYP450 1A5的表达，从而缓解AFB1诱导的原代肝细胞损伤。
外文摘要：This study aims to evaluate the protective effects of selenomethionine (SeMet) on Aflatoxin B1 (AFB1)-induced hepatotoxicity in primary chicken hepatocytes. Cell viability and lactic dehydrogenase activity assays revealed the dose-dependency of AFB1 toxicity to chicken hepatocytes. AFB1 concentrations above 0.05 μg/mL significantly reduced glutathione and total superoxide dismutase levels, as well as increased malondialdehyde concentration and cytochrome P450 enzyme 1A5 (CYP450 1A5) mRNA levels (P < 0.05). AFB1, however, did not affect CYP450 3A37 mRNA levels. Supplementation with 2 μM SeMet protected against AFB1-induced changes and significantly increased selenoprotein W (SelW) mRNA levels (P < 0.05). Additionally, SelW knockdown attenuated the protective effect of SeMet on AFB1-induced damage and significantly increased CYP450 1A5 expression (P < 0.05). Therefore, SeMet alleviates AFB1-induced damage in primary chicken hepatocytes by improving SelW expression, thus inhibiting CYP450 1A5 expression.
外文关键词：selenomethionine；AFB1；cytochrome P450 enzymes；siRNA；selenoprotein W；primary chicken hepatocytes
作者：Chen, XX；Che, CP；Korolchuk, VI；Gan, F；Pant, CL；Huang, KH
作者单位：Nanjing Agr Univ
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：2.857
出版年份：2017
出版刊次：12
点击下载：硒代蛋氨酸通过上调SelW的表达抑制CYP450 1A5的表达来缓解鸡原代肝细胞中AFB1诱导的损伤