中文摘要：真菌毒素是存在于食物中的次生真菌代谢物，会对人体和动物产生不良影响。本研究旨在开发和验证多霉菌毒素方法，以确定水稻、玉米制品和小麦制品中的黄曲霉毒素、黄绿青霉素、脱氧雪腐镰刀菌烯醇、伏马毒素、赭曲霉毒素A、玉米赤霉烯酮及某些代谢物/衍生物，以及玉米制品中的总伏马毒素（游离和结合/隐藏形式）。
外文摘要：Mycotoxins are secondary fungi metabolites present in foods that cause adverse effects in humans and animals. The aims of this work were to develop and validate a multi-mycotoxin method to determine the presence of aflatoxins, citreoviridin, deoxynivalenol, fumonisins ,ochratoxin A, zearalenone and some metabolites/derivatives in rice, maize-based products and wheat-based products, and a method to determine total fumonisins (free and bound/hidden forms) in maize-based products. The validated multi-mycotoxin method was based on extraction with acidified acetonitrile and LC-ESI+-MS/MS analysis, with LOQs ranging from 0.5 to 121μg/kg, and proved to be suitable for the multi-mycotoxin analysis in wheat, maize and rice products. Bound/hidden fumonisins were determined after extraction of the free forms using the multi-mycotoxin method, followed by a basic hydrolysis of the unextracted bound/hidden and solid-liquid extraction with low temperature purification (SLE-LTP). The hydrolysis efficiency of the bound/hidden extraction procedure was investigated by analyzing a maize reference material and showed recoveries ranging from 75% (HFB2) to 108% (HFB1). The use of isotope internal standards was crucial for mycotoxins quantification in maize meal and wheat flour, while for rice, external calibration and matrix matched curves gave satisfactory results. All 55 samples of wheat-based products analyzed were contaminated with at least one mycotoxin and 16% of 44 rice samples were also contaminated. The most prevalent mycotoxins were DON and ZON in wheat-based products.
 
外文关键词：Mycotoxins；LC-MS/MS；Cereals；Bound fumonisins；Isotope internal standard
作者：Andradel, PD；Dantas, RR；de Moura-Alves, TLD；Caldas, ED
作者单位：Univ Brasilia
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：1.494
出版年份：2017
出版刊次：3
点击下载：利用同位素内标法和正离子模式液相色谱/串联质谱法测定谷物中的多种霉菌毒素和玉米制品中的总伏马菌素