中文摘要：禾谷镰刀菌是全球一种严重病害，小麦赤霉病的主要病因。亚油酸异构酶（LAI）能够催化亚油酸（LA）转变为对人体健康有益的共轭亚油酸（CLA）。本研究表征了被一种植物防御激素水杨酸下调的禾谷镰刀菌的cis-12 LAI基因(FGSG_ 02668; FgLAI12)。小麦真菌中FgLAI12基因编码的cis-12 LAI酶对水杨酸、菌丝体生长和毒性的反应至关重要。 FgLAI12基因对于cis-9、trans-11 CLA的生物合成可能是有价值的。
外文摘要：Fusarium graminearum is the major causal agent of fusarium head blight in wheat, a serious disease worldwide. Linoleic acid isomerase (LAI) catalyses the transformation of linoleic acid (LA) to conjugated linoleic acid (CLA), which is beneficial for human health. We characterised a cis-12 LAI gene of F. graminearum (FGSG_ 02668; FgLAI12), which was downregulated by salicylic acid (SA), a plant defence hormone. Di0sruption of FgLAI12 in F. graminearum resulted in decreased accumulation of cis-9, trans-11 CLA, enhanced sensitivity to SA, and increased accumulation of LA and SA in wheat spikes during infection. In addition, mycelial growth, accumulation of deoxynivalenol, and pathogenicity in wheat spikes were reduced. Re-introduction of a functional FgLAI12 gene into Delta FgLAI12 recovered the wild-type phenotype. Fluorescent microscopic analysis showed that FgLAI12 protein was usually expressed in the septa zone of conidia and the vacuole of hyphae, but was expressed in the cell membrane of hyphae in response to exogenous LA, which may be an element of LA metabolism during infection by F. graminearum. The cis-12 LAI enzyme encoded by FgLAI12 is critical for fungal response to SA, mycelial growth and virulence in wheat. The gene FgLAI12 is potentially valuable for biotechnological synthesis of cis-9, trans-11 CLA.
外文关键词：FUNGUS MORTIERELLA-ALPINA; HEAD BLIGHT; HETEROLOGOUS
作者：Zhang, YZ；Wei, ZZ；Liu, CH；Chen, Q；Xu, BJ；Guo, ZR
作者单位：Sichuan Agr Univ
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.259
出版年份：2017
出版刊次：-
点击下载：亚油酸异构酶基因FgLAI12影响禾谷镰刀菌对水杨酸的敏感性、菌丝体生长以及毒性