中文摘要：本文研究了两种生物防治剂（粉红粘帚霉，BCA1；革兰氏阴性细菌，BCA5）控制不同成熟期：乳熟期，R-3；蜡熟期，R-4和齿期，R-5）玉米棒中轮状镰刀菌FV1的FUM1基因表达及伏马菌素产生的功效。结果显示，拮抗剂对成熟玉米棒子中霉菌毒素生产的控制功效需要考虑到病原菌和拮抗剂的生态生理学，以及玉米抽丝期的生理状态，以确保有效地控制。
外文摘要：This study was carried out to examine the efficacy of two biocontrol agents (Clonostachys rosea 016, BCA1; Gramnegative bacterium, BCA5) for control of FUM1 gene expression and fumonisin B-1 (FB1) production by F. verticillioides FV1 on maize cobs of different ripening stages: R-3, Milk (0.985 a(w)); R-4, Dough (0.976 a(w)); R-5, Dent (0.958 a(w)). Initially, temporal studies on FUM1 gene expression and FB1 production were performed on maize kernels for up to 14 days. This revealed that day 10 was optimum for both parameters, and was used in the biocontrol studies. Maize cobs were inoculated with 50:50 mixtures of the pathogen:antagonist inoculum and incubated in environmental chambers to maintain the natural a(w) conditions for ten days at 25 and 30 degrees C. The growth rates of F. verticillioides FV1, the relative expression of the FUM1 gene and FB1 production were quantified. It was found that, a(w) x temp had significant impacts on growth, FUM1 gene expression and FB1 production by F. verticillioides FVI on maize cobs of different maturities. The fungal antagonist (BCA1) significantly reduced FB1 contamination on maize cobs by>70% at 25 degrees C, and almost 60% at 30 degrees C regardless of maize ripening stage. For the bacterial antagonist (BCA5) however, FB1 levels on maize cobs were significantly decreased only in some treatments. These results suggest that efficacy of antagonists to control mycotoxin production in ripening maize cobs needs to take account of the ecophysiology of the pathogen and the antagonists, as well as the physiological status of the maize during silking to ensure effective control.
外文关键词：Biocontrol; Fusarium; Fumonisin B-1; FUM genes; Maize cob; Maturity stages; Silking
作者：Samsudin, NIP；Rodriguez, A；Medina, A；Magan, N
作者单位：Cranfield Univ
期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
期刊影响因子：3.339
出版年份：2017
出版刊次：-
点击下载：真菌和细菌拮抗剂控制不同成熟期玉米棒中轮状镰刀菌的生长、FUM1基因表达和伏马菌素B=1生产的功效