中文摘要：本研究利用响应面法优化了用红串红球菌去除榨油后的残渣，花生饼中的黄曲霉毒素B1。研究发现还需要进行进一步、更大规模的研究，检验所得结果，并且通过动物实验完成最终产品的安全性评估。
外文摘要：Degradation of aflatoxin B-1 in groundnut cake, a residue after oil extraction, by Rhodococcus erythropolis was optimized using response surface methodology. The bacterium was first grown in synthetic medium to determine optimal growth conditions using the combination of the Plackett-Burman and Box-Behnken methods and consequently aflatoxin B-1 in groundnut cake slurry was degraded under varying culture conditions. Optimal growth conditions were found as 22.5 A degrees C of temperature, pH 7, 100 mL of culture volume in 500 mL flasks, 1 % (v/v) of inoculum size, 135 rpm of agitation speed, 5 g/L of glucose and 5 g/L of peptone concentration according to analysis of the Box-Behnken design. Optimal detoxification conditions were found as 27.4 % (w/v) of solid concentration, 4.88 % (v/v) of inoculum size and 24 h of incubation time by Box-Behnken response surface optimization. Maximum detoxification level was predicted as 92.2 % by the constructed model while the experimental counterpart was 87.3 %. The suggested culture conditions have the potential to decrease aflatoxin B-1 from 200 mu g/kg to below 20 mu g/kg, the regulatory limit, in feed materials. Further studies are needed to test the obtained results at a larger scale and to finalize safety assessment of the final product by animal testing.
外文关键词：Mycotoxins; Aflatoxin B-1; Detoxification; Rhodococcus erythropolis; Groundnut cake; Fermentation
作者：Dogan, OB；Onal-Ulusoy, B；Bozoglu, F；Sagdicoglu-Celep, AG；Cekmecelioglu, D
作者单位：Middle East Tech Univ
期刊名称：WASTE AND BIOMASS VALORIZATION
期刊影响因子：1.337
出版年份：2017
出版刊次：3
点击下载：在摇瓶生物反应器中用红串红球菌对受AFB1自然污染的花生饼进行解毒