中文摘要：对于牛奶中黄曲霉毒素M1（AFM1）和氯霉素（CAP）浓度的严格要求已经开始慢慢显露了。本研究开发了一种基于背景荧光猝灭的免疫层析法（bFQICA）用于检测牛奶中的AFM1和CAP。用该方法检测牛奶中的AFM1和CAP非常方便，测试时间约为8分钟。另外，系统提供内部WiFi设施，允许在智能电话中快速连接和存储。
外文摘要：Harsh demanding has been exposed on the concentration of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk. In this study, we developed a new method based on background fluorescence quenching immunochromatographic assay (bFQICA) to detect AFM1 and CAP in milk. The detection limit for AFM1 was 0.0009 ng/mL, while that for the CAP was 0.0008 ng/mL. The assay variability was determined with 3 AFM1 standards (i.e., 0.25 ng/mL, 0.5 ng/mL, and 1.0 ng/mL), and the actual detection value was 0.2497, 0.5329, and 1.0941, respectively. For the assay variability of 3 CAP standards (i.e., 0.10 ng/mL, 0.30 ng/mL, and 0.50 ng/mL), the actual detection value was 0.0996, 0.3096, and 0.4905, respectively. The recovery rate of AFM1 was 99.7%-101.7%, while that for CAP was 95.3%-97.6%. For the test stability, AFM1 and CAP showed satisfactory test stability even at month 5. Compared with the sensitivity of liquid chromatography-mass spectrometry (LC-MS) method,no statistical difference was noticed in results of the bFQICA. Our method is convenient for the detection of AFM1 and CAP in milk with a test duration of about 8 minutes. Additionally, an internal WiFi facility is provided in the system allowing for quick connection and storage in the intelligent cell phone.
外文关键词：SUSPENSION ARRAY TECHNOLOGY；ELISA
作者：Wu,XX；Tian,XF；Xu,LH；Li,JT；Li,XX；Wang,YW
作者单位：Xinjiang Medical University
期刊名称：BIOMED RESEARCH INTERNATIONAL
期刊影响因子：2.134
出版年份：2017
出版刊次：10
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