中文摘要：提高小分子（半抗原）浓度测量的灵敏度和重现性对于其广泛的应该至关重要。通常用竞争性分析，它对传感芯片的吸附能力及固定在表面的共轭物中抗原决定簇的可及性的依赖性很强，来实现用不同的生物传感器免疫化学检测半抗原。本文提出了一种考虑传感区域内特异吸附能力，提高竞争性分析的重现性，从而明显改善半抗原无标记检测限的方法。该方法大幅提高了竞争性分析的重现性，可用于其它任何无标记生物传感器。这一特性与已知的无标记生物传感器的优点相结合，在食品分析、医学、生物安全和环境监测等领域中，对小分子的精确、快速、灵敏和多重检测具有吸引力。
外文摘要：Enhancement of sensitivity and reproducibility of measuring the concentration of small molecules (haptens) is crucially important for a wide variety of applications. The immunochemical detection of haptens by different biosensors is commonly realized by competitive assays that strongly depend on sorption capacity of the sensor chip and accessibility of antigen determinants in a conjugate immobilized on its surface. Here, we propose a method for significant improvement of limits of label-free detection of haptens due to enhancement of reproducibility of the competitive assay by taking into account the specific sorption capacity within the sensing area. The method is validated by mycotoxin detection in complex food matrices such as white wine. The limits of detection of 0.25 ng/mL, 0.48 ng/mL and 1 ng/mL are achieved for ochratoxin A, zearalenone, and aflatoxin B1, respectively, using compact label-free interferometric biosensors that employ microscope cover slips as affordable single-used sensor chips. The proposed method of drastic improvement of reproducibility of competitive assays can be used by any other label-free biosensors. This feature combined with the well-known advantages of label-free biosensors could make them attractive for precise, rapid, sensitive and multiplex detection of small molecules in food analysis, medicine, biosafety and environmental monitoring, etc.
外文关键词：Label-free optical biosensor；Interferometry；Competitive immunoassay；Self-reference method；Mycotoxin；Ochratoxin A；Zearalenone；Aflatoxin B1；Food control
作者：Orlov, AV；Burenin, AG；Massarskaya, NG；Betin, AV；Nikitin, MP；Nikitin, PI
作者单位：Russian Acad Sci
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：4.758
出版年份：2017
出版刊次：7
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