中文摘要：展青霉素是污染苹果的主要霉菌毒素，在酒精饮料的酿造过程中，降解为ascladiol，它也是展青霉素最后的前体。本研究目的有二：（1）表征展青霉素生物合成途径的最后一步和（2）研究ascladiol的毒性。研究表明E-和Z-ascladiol无毒，因此展青霉素解毒生成ascladiol是限制展青霉素风险的好办法。
外文摘要：Patulin is the main mycotoxin contaminating apples. During the brewing of alcoholic beverages, this mycotoxin is degraded to ascladiol, which is also the last precursor of patulin. The present study aims (1) to characterize the last step of the patulin biosynthetic pathway and (2) to describe the toxicity of ascladiol. A patE deletion mutant was generated in Penicillium expansum. In contrast to the wild strain, this mutant does not produce patulin but accumulates high levels of E-ascladiol with few traces of Z-ascladiol. This confirms that patE encodes the patulin synthase involved in the conversion of E-ascladiol to patulin. After purification, cytotoxicities of patulin and E- and Z-ascladiol were investigated on human cell lines from liver, kidney, intestine, and immune system. Patulin was cytotoxic for these four cell lines in a dose-dependent manner. By contrast, both E- and Z-ascladiol were devoid of cytotoxicity. Microarray analyses on human intestinal cells treated with patulin and E-ascladiol showed that the latter, unlike patulin, did not alter the whole human transcription. These results demonstrate that E- and Z-ascladiol are not toxic and therefore patulin detoxification strategies leading to the accumulation of ascladiol are good approaches to limit the patulin risk.
外文关键词：Ascladiol；Patulin synthase；Penicillium expansum；patE gene；Cytotoxicity；Microarray analysis
作者：Tannous, J；Snini, SP；El Khoury, R；Canlet, C；Pinton, P；Lippi, Y；Alassane-Kpembi, I；Gauthier, T；El Khoury, A；Atoui, A；Zhou, T；Lteif, R；Oswald, IP；Puel, O
作者单位：Univ Toulouse
期刊名称：ARCHIVES OF TOXICOLOGY
期刊影响因子：6.637
出版年份：2017
出版刊次：6
点击下载：展青霉素转化产物及其生物合成途径中最后的中间体，E-和Z-ascladiol，对人体细胞无毒