中文摘要：本研究开发了一种基于磁性纳米粒子(MNPs)的快速灵敏的化学发光免疫分析法(CLIA)，用来检测黄曲霉毒素B1(AFB1)，一种天然的强致癌物。
外文摘要：A rapid and sensitive chemiluminescence immunoassay (CLIA) based on magnetic nanoparticles (MNPs) was developed to detect aflatoxin B1 (AFB1), which is a potent carcinogen in nature. We prepared monodisperse MNPs (300 nm in diameter) according to the solvothermal synthesis reaction and the MNPs were coated with silica by the Stöber method. Triethox was used as a one-step carboxylation reagent, and 3-aminopropyltriethoxysilane (APTES) an amination reagent, to modify the MNPs. We prepared two types of solid phase antigens using the above synthesized functionalized MNPs coupled with the later prepared AFB1-oxime active ester and the purchased BSA-AFB1 respectively. 2′,6′-dimethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4′-N-hydroxysuccinimide (4′-NHS) ester (NSP-DMAE-NHS), as a novel luminescent reagent, was used to label anti-AFB1 antibodies. The two CLIA calibration curves based on the two types of solid phase antigens were obtained and compared. The acquired limit of detection (LOD) was about 0.001 ng/mL for the two functionalized MNPs-based immunoassays, and the half maximal inhibitory concentration (IC50) was 0.51 ng/mL for the MNPs-AFB1-based method and 0.72 ng/mL for the MNPs-BSA-AFB1-based method.
外文关键词：AFB1 detection；chemiluminescent immunoassays (CLIA)；functionalization；magnetic nanoparticles；NSP-DMAE-NHS
作者：Yao, MW；Wang, LY；Fang, CZ
作者单位：Tongji Univ
期刊名称：LUMINESCENCE
期刊影响因子：1.452
出版年份：2017
出版刊次：4
点击下载：用功能化的磁性纳米粒子结合两种抗原探针固定策略的化学发光免疫分析法检测黄曲霉毒素B１