中文摘要:本研究全面系统地比较了用荧光微球免疫层析法(FM-ICA)和量子点亚微球免疫层析法(QBICA)定量检测牛奶中黄曲霉毒素M1的性能。结果发现本研究可以作为一个用免疫层析法检测黄曲霉毒素M1时选择合适荧光标记的参考。
外文摘要:The performance of fluorescent microsphere immunochromatographic assay (FM-ICA) and quantum-dot submicrobead immunochromatographic assay (QBICA) was systematically and comprehensively compared in quantitative detection of aflatoxin M-1 in milk. Under optimum conditions, the advantages of FM-ICA include lower limit of detection of 42.3 pg/mL with better accuracy, precision, reliability, and practicability. The advantages of QB-ICA include shorter detection time and lower monoclonal antibody consumption. The 2 ICA were consistent with liquid chromatography tandem mass spectrometry. This study serves as a reference for selecting appropriate fluorescent labels for the immunochromatographic assay of aflatoxin M-1.
外文关键词:immunochromatographic assay; fluorescent microsphere; quantum-dot submicrobead; milk
作者:Wu, CH;Hu, LM;Xia, J;Xu, GM
作者单位:Nanchang University
期刊名称:JOURNAL OF DAIRY SCIENCE
期刊影响因子:2.408
出版年份:2017
出版刊次:4
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