中文摘要：黄曲霉毒素是一种有毒且致癌的化合物，常见于作物和植物体内。黄曲霉毒素B1是黄曲霉毒素中毒性最强的化合物，被列入一级致癌物，尤其是肝癌。本研究开发出一种环境质谱方法用于花生中黄曲霉毒素B1的快速表征。研究表明，直接探针电喷雾质谱（DEP-MS/MS）结合简单的分散液-液微萃取步骤能够成功应用于花生样本中AFB1的定量分析。该方法效率很高，有望为现实生活中的食品安全提供重要的毒理信息。
外文摘要：Rationale Aflatoxins are poisonous and cancer-related chemical compounds commonly found in crops and plants. Aflatoxin B-1 is the most toxic compound among aflatoxins and has been classified as group 1 carcinogenic to humans, especially in liver cancer. Herein, an ambient mass spectrometric method was developed for rapid characterization of trace aflatoxin B-1 in peanuts.
Methods Direct electrospray probe tandem mass spectrometry (DEP-MS/MS) was used to detect aflatoxin B-1 in peanuts. To avoid the matrix effect, the aflatoxin B-1 in the samples was extracted and concentrated by dispersive liquid-liquid micro extraction. The mass spectrometer was operated in the positive ion mode to monitor the intact molecular ion (m/z 313, MH+) and product ion (m/z 241) of aflatoxin B-1 using multiple reaction monitoring.
Results Since no clean-up procedure of the sample was required, the sampling step and the subsequent mass spectrometric detection of the aflatoxin B-1 was completed in less than 5min. The limit of detection of aflatoxin B-1 is at the sub-ppb level. The results obtained by DEP-MS/MS were also validated by liquid chromatography/tandem mass spectrometry (LC/MS/MS). Recovery of aflatoxin B-1 in the sample was evaluated by analyzing spiked aflatoxin B-1 with LC/MS/MS to be 85% and DEP-MS/MS to be 84%.
Conclusions DEP-MS/MS combined with a simple dispersive liquid-liquid micro extraction procedure was successfully used for the quantitative analysis of AFB(1) in nut samples. Due to its high efficiency, it is promising in providing important toxicological information for food safety in the real world.
外文关键词：IMMUNOAFFINITY COLUMN CLEANUP; THIN-LAYER CHROMATOGRAPHY; G(1); FOOD; G(2); CONTAMINATION; EXTRACTION; PRODUCTS; PEANUTS; ASSAY
作者：Satarpai, T；Siripinyanond, A；Su, H；Shiea, J
作者单位：National Sun Yat Sen University
期刊名称：RAPID COMMUNICATIONS IN MASS SPECTROMETRY
期刊影响因子：2.226
出版年份：2017
出版刊次：8
点击下载：通过分散液-液微萃取以及直接探针电喷雾串联质谱法快速表征花生、小麦和玉米中的黄曲霉毒素B1