中文摘要：霉菌毒素可能污染花生和花生产品，是人类多种疾病的病因。黄曲霉毒素B1（AFB1）、G1（AFG1）、B2（AFB2）、G2（AFG2）、赭曲霉毒素A（OTA）和玉米烯酮（ZEN）是食物中最常见的霉菌毒素群。本研究旨在开发一种高效液相色谱结合荧光探测器（HPLC-FLD）联合分散液-液微萃取（DLLME）的方法用于花生中六种霉菌毒素的同步检测。本研究提出的方法简单、选择性高、精确、可靠，已成功应用于我国花生样品中六种霉菌毒素的同步检测。
外文摘要：BACKGROUND Mycotoxins, which may contaminate peanut and peanut products, are responsible for many diseases to humans. Aflatoxin B1 (AFB1), aflatoxin G1 (AFG1), aflatoxin B2 (AFB2), aflatoxin G2 (AFG2), ochratoxin A (OTA) and zearalenone (ZEN) are considered the most relevant groups of mycotoxins found in food. This work aimed to develop a high-performance liquid chromatography method with a fluorescence detector (HPLC-FLD) combined with dispersive liquid-liquid microextraction (DLLME) method for the simultaneous determination of the six mycotoxins in peanuts. The six mycotoxins were simultaneously determined under their best wavelength by means of changing wavelength.
RESULTS Under the optimum conditions, the linear ranges were 1-100 ng mL(-1) for AFB1, AFG1 and OTA, 0.3-30 ng mL(-1) for AFB2 and AFG2, 5-1000 ng mL(-1) for ZEN, with the correlation coefficient (R-2) of 0.9969-0.9997. Limits of detection (LODs) were 0.10, 0.10, 0.30, 0.03, 0.03 and 1.0 mu g kg(-1), respectively, and the mean recoveries were in the range of 83.1% to 99.3% with RSD < 10% (n = 6, independent analysis). Thirteen (46%) of these tested samples were contaminated with at least one mycotoxin.
CONCLUSION The proposed method was demonstrated to be simple, highly selective, accurate, reliable, and was successfully applied to simultaneously analyse the six mycotoxins in real peanut samples from China.
外文关键词：simultaneous determination; six mycotoxins; HPLC-FLD; peanut
作者：Chen, Fangfang; Luan, Chuanlei; Wang, Lin
作者单位：Shandong University
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.076
出版年份：2017
出版刊次：6
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