中文摘要：本研究开发了一种快速检测小麦样品中玉米赤霉烯酮(ZEN)的胶体金(ICS)试纸。研究表明ICS试纸可用作现场定性检测ZEN的工具。
外文摘要：A colloidal gold  test was developed for rapid detection of zearalenone (ZEN) in wheat samples. The mAb against ZEN was prepared in our laboratory and labelled with colloidal gold as a probe for the ICS test. The conditions were optimized and 30 nm colloidal gold nanoparticles were chosen for optimal performance. Millipore 135 was chosen as the NC membrane for its level of sensitivity. The optimum amount of coated antigen ZEN-OVA and anti-ZEN mAb was 0.5 mg/mL and 8 μg/mL, respectively. The ICS test, which has a detection limit of 15 ng/mL for ZEN, could be completed in 5 min. Analysis of ZEN in 202 wheat samples over three consecutive years revealed that data obtained from the ICS test were in a good agreement with LC-MS/MS data. This result demonstrated that the ICS test could be used as a qualitative tool to screen on-site for ZEN.
作者：Ji, F; Mokoena, MP; Zhao, HY; Olaniran, AO; Shi, JR
作者单位：Jiangsu Acad Agr Sci
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2017
出版刊次：5
点击下载：开发一种免疫层析试纸条快速检测我国江苏省小麦中的玉米赤霉烯酮