中文摘要：为了研究一种灵敏的筛选玉米中的玉米赤霉烯酮类真菌毒素的荧光偏振免疫分析方法（FPIA），本研究制备了两种与玉米赤霉烯酮类有均一亲和力的新型单克隆抗体（MAb）和四种荧光素标记的示踪剂。从灵敏性和特异性方面仔细选择合适的示踪剂-抗体进行配对后，研究出一种可以同时检测具有类似灵敏度的玉米赤霉烯酮类的FPIA。用FPIA筛选自然污染的玉米样品，其结果与高效液相色谱-串联质谱法有良好的相关性。
外文摘要：To develop a sensitive fluorescence polarization immunoassay (FPIA) for screening the zearalenone class of mycotoxins in maize, two new monoclonal antibodies (MAbs) with uniform affinity to zearalenone class and four fluorescein-labeled tracers were prepared. After careful selection of appropriate tracer-antibody pairs in term of sensitivity and specificity, a FPIA that could simultaneously detect zearalenone class with similar sensitivity was developed. Under optimum conditions, the half maximal inhibitory concentrations (IC50) of the FPIA in buffer were 1.89, 1.97, 2.43, 1.99, 2.27 and 2.44 μg/L for zearalenone, α-zearalenol, β-zearalenol, α-zearalanol, β-zearalanol and zearalanone, respectively. The limit of detection of FPIA for the zearalenone class was around 12 μg/kg in maize and the recoveries ranged from 84.6-113.8% with coefficients of variation below 15.3% in spiked samples. Finally, the FPIA was applied for screening naturally contaminated maize samples and the results indicated a good correlation with that of high-performance liquid chromatography-tandem mass spectrometry.
外文关键词：zearalenone；monoclonal antibody；fluorescence polarization immunoassay；maize；detection
作者：Zhang, XY；Eremin, SA；Wen, K；Yu, XZ；Li, CL；Ke, YB；Jiang, HY；Shen,JZ；Wang, ZH
作者单位：China Agr Univ
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：2.857
出版年份：2017
出版刊次：10
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