中文摘要：黄曲霉毒素B1（AFB1），一种肝致癌物及高毒性霉菌毒素，是粮食的污染物，特别是炎热潮湿的气候对产黄曲霉毒素的真菌的生长有利。因孟加拉缺乏AFB1污染食品及饲料的资料，本研究采用ELISA初步筛选了孟加拉人尿液中代谢物及生物标志物黄曲霉毒素M1(AFM1)的存在情况，揭示出频繁的膳食暴露。对比其它国家的生物标志物数据，孟加拉人的检出频率及尿液中AFM1的含量引发了对强致癌物黄曲霉毒素的关注。
外文摘要：Aflatoxin B1 (AFB1), a hepatocarcinogen and highly toxic mycotoxin, is a contaminant of food commodities, especially in hot and humid climates that favour the growth of aflatoxin-producing fungi. As data on AFB1 contamination of food and feed in Bangladesh are scarce, we conducted an initial screening by ELISA on the occurrence of the metabolite and biomarker aflatoxin M1 (AFM1) in urines from Bangladesh which indicated frequent exposure. This finding led us to conduct a follow-up study where we applied a more sensitive method (IAC clean-up and HPLC-FD analysis) to determine AFM1 concentrations in a larger set of urine samples. To account for possible seasonal and regional differences in mycotoxin exposure, in total 218 urines were collected in two districts of Bangladesh: 164 urines (n = 69 in summer, n = 95 in winter) from residents of a rural and an urban area in Rajshahi district, among them 62 participants enrolled in both sampling periods, and 54 urine samples obtained from pregnant women in Dhaka district. AFM1 was detected in >40% of all Rajshahi urine samples at a range of 1.7–104 pg/mL in summer and at a range of 1.8–190 pg/mL in winter season. The mean level of urinary AFM1 was higher in winter (27.7 ± 42.6 pg/mL) than in summer (13.6 ± 21.2 pg/mL) season, and differences were observed at the mean AFM1 level between the rural and the urban Rajshahi cohort. AFM1 was found less frequently in the Dhaka pregnant women (31% above LOD, mean 13.9 ± 33.3 pg/mL), but in a similar concentration range (1.7–141 pg/mL) as in the Rajshahi cohort. Urinary AFM1 levels did not show significant associations with the participants food consumption pattern. In conclusion, when compared to biomarker data from other countries, detection frequency and urinary AFM1 levels in our Bangladeshi cohorts raise concerns regarding their exposure to potent carcinogenic aflatoxins.
外文关键词：Aflatoxins；Biomarkers；Cohorts；Exposure；Urine
作者：Ali, N；Blaszkewicz, M；Hossain, K；Degen, GH
作者单位：TU Dortmund
期刊名称：INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
期刊影响因子：3.98
出版年份：2017
出版刊次：2
点击下载：尿样中黄曲霉毒素M1的检测揭示了孟加拉人频繁的黄曲霉毒素B1膳食暴露