中文摘要：营养成分对转基因作物的安全性评估来说是非常重要的。三种富含β-胡萝卜素的转基因大豆植株在开发时均被引入了β-伴大豆球蛋白启动子。本文在含有170.47-213.58 mu g/gβ-胡萝卜素的产品中成功地检测到转基因大豆，并对3种转基因大豆、非转基因大豆和几个商品大豆进行了营养成分比较评价。研究发现，转基因大豆与其对照组的大多数成分（如脂肪酸、氨基酸、维生素等）相似。然而，转基因大豆与非转基因大豆的粗脂肪、碳水化合物、δ生育酚和油酸的水平差异非常的显著。营养成分的改变需要做进一步的转录组和代谢组分析来确定。
外文摘要：Nutritional composition is important for assessing the safety of genetically modified (GM) crops for human consumption. Three beta-carotene-enhanced soybean lines were developed by introducing the beta-conglycinin promoter:: Phytoene synthase-2A-Carotene desaturase/t35S gene cassette into the genome of the commercial Kwangan (Glycine max L.) soybean variety. Transgenic soybeans were successfully detected on beta-carotene productions ranged from 170.47 to 213.58 mu g/g. Comparative assessments of nutrition were conducted with 3 transgenic soybeans, their non-GM counterpart, and several commercial soybean varieties. Results indicated that most levels of proximate, fatty acids, amino acids, and vitamins showed non-significant differences between transgenic soybeans and their counterpart, and fit within the reference ranges established for other commercial soybeans and Organization for Economic Cooperation and Development Guidelines. However, significant differences on levels of crude fat, carbohydrate, delta-tocopherol, and oleic acid of transgenic soybeans comparing to those of non-transgenic counterpart Kwangan cannot eliminate the influences of transgene insertion. Alternations on compositions should be definite by further studies, such as transcriptome and metabolome profiling.
外文关键词：Beta-carotene enhanced; Genetically modified; Nutritional composition; Soybean (Glycine max L.); Substantial equivalence
作者：Qin, Yang; Park, Soo-Yun; Oh, Seon-Woo; 等
作者单位：Natl Inst Agr Sci
期刊名称：APPLIED BIOLOGICAL CHEMISTRY
期刊影响因子：1.751
出版年份：2017
出版刊次：7
点击下载：富含β-胡萝卜素的转基因大豆的营养成分分析