中文摘要：薄膜芯片的反向斑点杂交技术RDBH已被用于检测有害物质，但自动薄膜芯片RDBH设备的低负荷、高精度和稳定性仍然是必要的。本文介绍了一种新开发的用于转基因玉米多重筛选的自动微流控薄膜芯片方法（AMTC）。研究发现，AMTC方法对转基因玉米的检测灵敏度达到0.1%，且稳定性和一致性均能满足实际应用的要求。另外，ATMC方法的性能优势是多方面的，如操作容易简单、成本低和工作量少等。
外文摘要：As a thin-film chip method, reverse dot blot hybridization (RDBH) has been employed to detect hazardous substances, but an automatic RDBH instrument with low workload, high accuracy and stability is still urgently needed. This paper presents our newly-developed auto-microfluidic thin-film chip (AMTC) method for multiplex screening of genetically modified (GM) maize. With specific DNA probes for genetically modified (GM) maize being immobilized on a square nylon thin-film, it was placed into a micro-reaction cell of the AMTC device. Then biotin-labeled PCR products with target DNA fragments for template amplification were added to the micro-reaction cell using a microfluidic system. When the PCR products passed the square nylon thin-film, the target DNA fragments were captured by the complementary action of DNA, where the signal was visualized with streptavidin link-coupled alkaline phosphatase color development kit. The sensitivity of GM maize detection reached 0.1% quality percentage and its stability and consistency could satisfy the requirements for practical applications. Performance advantages of the ATMC are manifold, being embodied in aspects such as easy and straightforward operation, low costs and less workload. 
外文关键词：Thin-film chip; Reverse dot blot hybridization; Genetically modified organism; Microfluidic; Detection
作者：Yun, Zhenyu; Peng, Liping; Huo, Shengnan; 等.
作者单位：Sichuan Hua Hansan Bio Technol Co Ltd
期刊名称：FOOD CONTROL
期刊影响因子：3.496
出版年份：2017
出版刊次：10
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