中文摘要：为了尽量减少花生种子中的黄曲霉毒素污染，人们付出了巨大的努力。一种可能的策略是了解并利用植物抵抗真菌感染的机制。本研究在生物化学和分子水平上识别并表征了花生抗性材料PI 337394和易感品种Forman的种子在寄生曲霉定植期产生的植物蛋白酶抑制剂（PPIs）。分析了PPI在种子抵抗真菌感染中的生物学意义，并与感染期间真菌释放的酶的抑制特性以及KPI的抗真菌效果联系起来。
外文摘要：Significant efforts are being made to minimize aflatoxin contamination in peanut seeds and one possible strategy is to understand and exploit the mechanisms of plant defense against fungal infection. In this study we have identified and characterized, at biochemical and molecular levels, plant protease inhibitors (PPIs) produced in peanut seeds of the resistant PI 337394 and the susceptible Forman cultivar during Aspergillus parasiticus colonization. With chromatographic methods and 2D-electrophoresis-mass spectrometry we have isolated and identified four variants of Bowman-Birk trypsin inhibitor (BBTI) and a novel Kunitz-type protease inhibitor (KPI) produced in response to A. parasiticus colonization. KPI was detected only in the resistant cultivar, while BBTI was produced in the resistant cultivar in a higher concentration than susceptible cultivar and with different isoforms. The kinetic expression of KPI and BBTI genes along with trypsin inhibitory activity was analyzed in both cultivars during infection. In the susceptible cultivar an early PPI activity response was associated with BBTI occurrence. Meanwhile, in the resistant cultivar a later response with a larger increase in PPI activity was associated with BBTI and KPI occurrence. The biological significance of PPI in seed defense against fungal infection was analyzed and linked to inhibitory properties on enzymes released by the fungus during infection, and to the antifungal effect of KPI.
外文关键词：TRYPSIN-INHIBITOR；ARACHIS-HYPOGAEA；PROTEINASE-INHIBITOR；ANTIFUNGAL PROTEIN；INFECTION；EXPRESSION；CYSTEINE；FLAVUS；GENES；MAIZE
作者：Muller, V；Bonacci, G；Batthyany, C；Ame, MV
作者单位：National University of Cordoba
期刊名称：PHYTOPATHOLOGY
期刊影响因子：3.011
出版年份：2017
出版刊次：4
点击下载：对寄生曲霉的定植有不同抗性的花生品种显示出蛋白酶抑制剂的时间差异反应