中文摘要：本研究共收集了119份花生样品，贯穿了巴西圣保罗的花生生产链。花生样品直接用于感染率测定，并用多相鉴定识别曲霉属Flavi组的种类。本研究报道的数据提供了花生生产链多个阶段中真菌的出现及生物多样性的信息。黄曲霉毒素的发生也与连续监测和评估消费者消费花生引起的黄曲霉毒素暴露非常相关。
外文摘要：A total of 119 samples of peanut were collected throughout the peanut production chain in Sao Paulo State, Brazil. The peanut samples were directly plated for determination of percentages  of infection and a polyphasic approach was used to identify Aspergillus section Flavi species. Further, the potential for aflatoxin production by the isolates was tested using the agar plug technique and the presence of aflatoxins in peanuts was assessed using an immunoaffinity column followed by quantification using HPLC with reverse phase column and fluorescence detection. The limit of detection and quantification were 0.05 and 0.17 mu g/kg for total aflatoxins, respectively. Four species of Aspergillus section Flavi were isolated: A. caelatus (11), A. flavus (515), A. parasiticus (17) and A. tamarii (13). All isolates of A. parasiticus were able to produce aflatoxin B and G whereas aflatoxin B was produced by 50% of A. flavus isolates. Aflatoxins were found in 12 samples at concentrations ranging from 0.3 to 100 mu g/kg. The data reported in this study add information on the occurrence and biodiversity of fungi in peanuts at several stages of the production chain. The occurrence of aflatoxins is also of major relevance for continuous monitoring and assessment of likely exposure of consumers to aflatoxins through consumption of peanuts.
外文关键词：Aspergillus section Flavi；Peanut chain；Aflatoxins
作者：Martins, LM；Sant'Ana, AS；Fungaro, MHP
作者单位：ITAL, Inst Food Technol
期刊名称：FOOD RESEARCH INTERNATIONAL
期刊影响因子：3.182
出版年份：2017
出版刊次：-
点击下载：巴西花生生产链中曲霉属Flavi组和黄曲霉毒素的生物多样性