中文摘要：本研究用与常规相异的、经单一免疫亲和柱纯化样品改良的液相色谱-串联质谱(LC/MS/MS)法，同步检测谷物中的13种霉菌毒素（脱氧雪腐镰刀菌烯醇、雪腐镰刀菌醇、3-乙酰基雪腐镰刀菌烯醇、黄曲霉素B1、黄曲霉素B2、黄曲霉素G1、黄曲霉素G2、伏马菌素B1、伏马菌素B2、T-2、HT-2、玉米赤霉烯酮和赭曲霉毒素A）。该方法通过LC/MS/MS检测霉菌毒素具有较好的线性度、灵敏度、特异性和精确性。
外文摘要：An improved analytical method compared with conventional ones was developed for simultaneous determination of 13 mycotoxins (deoxynivalenol, nivalenol, 3-acetylnivalenol, aflatoxin B-1, aflatoxin B-2, aflatoxin G(1), aflatoxin G(2), fumonisin B-1, fumonisin B-2, T-2, HT-2, zearalenone, and ochratoxin A) in cereal grains by liquid chromatography-tandem mass spectrometry (LC/MS/MS) after a single immunoaffinity column clean-up. The method showed a good linearity, sensitivity, specificity, and accuracy in mycotoxin determination by LC/MS/MS. The levels of 13 mycotoxins in 5 types of commercial grains (brown rice, maize, millet, sorghum, and mixed cereal) from South Korea were determined in a total of 507 cereal grains. Mycotoxins produced from Fusarium sp. (fumonisins, deoxynivalenol, nivalenol, and zearalenone) were more frequently (more than 5%) and concurrently detected in all cereal grains along with higher mean levels (4.3-161.0 ng/g) in positive samples than other toxins such as aflatoxins and ochratoxin A (less than 9% and below 5.2 ng/g in positive samples) from other fungal species.
外文关键词：aflatoxins；deoxynivalenol；fumonisins；HT-2；nivalenol；ochratoxin A；T-2；zearalenone；grains；LC/MS/MS
作者：Kim, Dong-Ho；Hong, Sung-Yong；Kang, Jea Woo
作者单位：Korea University
期刊名称：TOXINS
期刊影响因子：3.029
出版年份：2017
出版刊次：3
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