中文摘要：伏马菌素是一种由镰刀菌（主要是再育镰刀菌和串珠镰刀菌）产生的霉菌毒素，对动物和人体健康有潜在危害。伏马菌素的毒性和作用机制还未确定，并且尚不清楚伏马菌素对昆虫细胞是否有毒。本文研究了伏马菌素B1（FB1）对草地贪夜蛾Sf9细胞系的毒性及其作用机制。本试验可以为其他种类昆虫细胞的伏马菌素胁迫研究提供参考。同时，也提供了一种用伏马菌素作生物杀虫剂前体化合物的可能性。
外文摘要：Fumonisins are a type of mycotoxin produced by Fusarium spp., mainly F. proliferatum and E vertieilliodes, and represent a potential hazard to the health of animals and human beings. The toxicity and mechanism of action of fumonisins is ambiguus, and it is unclear whether fumonisins are toxic to insect cells. This study examines the toxicity of fumonisin B1 (FB1) and its mechanism of action in the Spodoptera frugiperda Sf9 cell line. We found that FBI inhibited Sf9 cellular proliferation and arrested cell growth at the G2/M phase. Morphological observation showed that FBI induced swelling, vacuole formation, and loss of adhesion in Sf9 cells. Flow cytometry analysis showed that FBI caused depolarization of the cell membrane potential and hyperpolarization of the mitochondria] membrane potential. To uncover potential genes associated with the molecular mechanisms of FBI, 41 differentially expressed genes were identified by transcriptome analyses after FBI treatment. These genes are putatively involved in detoxification metabolism, insect hormone regulation, cell apoptosis, and other related processes. Finally, six differentially expressed genes were chosen and validated by quantitative real-time PCR (QRT-PCR). Our test could provide a reference for other kinds of insect cells studies on FBI stress. At the same time, our studies try to provide a possible for FB1 as a precursor compounds of biological insecticide
外文关键词：Fumonisin B-1；Sf9 cell lines；Toxicity；Genes
作者：Zhang, He；Zhang, Liyang；Diao, Xue
作者单位：South China Agr Univ
期刊名称：TOXICON
期刊影响因子：1.927
出版年份：2017
出版刊次：-
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