中文摘要：本研究旨在评估布拉氏酵母(S. boulardii)对脱氧雪腐镰刀菌烯醇(DON)诱导的猪肺泡巨噬细胞(PAMCs)损伤的作用并探索潜在的机制。结果表明布拉氏酵母能抑制DON诱导的p38 MAPK通路活化，减少下游炎症因子的表达，并促进抗凋亡基因的表达，从而抑制DON诱导的PAMC细胞凋亡。
外文摘要：The aims of our study were to evaluate the effects of Saccharomyces boulardii (S. boulardii) on deoxynivalenol (DON)-induced injury in porcine alveolar macrophage cells (PAMCs) and to explore the underlying mechanisms. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis, ELISA, qRT-PCR, and western blot were performed to assess whether S. boulardii could prevent DON-induced injury by p38 mitogen-activated protein kinase (p38 MAPK) signal pathway. The results showed that pretreatment with 8 μM DON could decrease the viability of PAMC and significantly increase the apoptosis rate of PAMC, whereas S. boulardii could rescue apoptotic PAMC cells induced by DON. Further experiments revealed that S. boulardii effectively reversed DON-induced cytotoxicity via downregulating the expression of TNF-α, IL-6, and IL-lβ. In addition, S. boulardii significantly alleviated DON-induced phosphorylation and mRNA expression of p38 and further increased the expression of apoptosis regulation genes Bcl-xl and Bcl-2 and inhibited the activation of Bax. Our results suggest that S. boulardii could suppress DON-induced p38 MAPK pathway activation and reduce the expression of downstream inflammatory cytokines, as well as promote the expression of anti-apoptotic genes to inhibit apoptosis induced by DON in PAMC.
外文关键词：DON, S. boulardii；p38 MAPK；mRNA expression；Cell apoptosis
作者：Chang, C；Wang, K；Zhou, SN；Wang, XD；Wu, JE
作者单位：Wuhan Polytech Univ
期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
期刊影响因子：1.606
出版年份：2017
出版刊次：1
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