中文摘要：T-2可以在动物组织中生物转化为改性T-2（mT-2s）。被T-2和/或mT-2s污染的食物对动物和人体，包括免疫系统都是有害的。在本研究中，口服饲喂凡纳滨对虾T-2毒素20天，并将肝胰脏和肌肉提取物T-2和T-2-葡糖苷酸（T-2-GluA）添加到体外培养的RAW264.7中，并研究它们对JAK / STAT通路的作用。研究表明，T-2和mT-2都具有细胞毒性，但T-2喂养的凡纳滨对虾的肝胰脏和肌肉提取物中，T-2对RAW264.7细胞中JAK / STAT通路的活化作用大于mT-2s。
外文摘要：T-2 can be biotransformed in animal tissues to modified T-2s (mT-2s). Food contaminated with T-2 and/or mT-2s is a hazard to both animals and humans, including the immune system. In this study, Litopenaeus vannamei were fed T-2 orally for 20 d, and hepatopancreas and muscle extracts, T-2, and T-2-glucuronide (T-2-GluA) were added to RAW264.7 in vitro and their effects on the JAK/STAT pathway were examined. STAT2 mRNA gene expression induced by hepatopancreas and muscle extracts was markedly higher compared with that of T-2 or T-2-GluA group. SCOSs, IL-6 and IL-1β mRNA gene expressions induced by hepatopancreas extract were greater than those induced by muscle extract. Muscle extract significantly activated STAT3 phosphorylation but inhibited STAT1 phosphorylation. Activation of the JAK/STAT pathway by hepatopancreas mT-2s was significantly higher than that by muscle extracts. Muscle and hepatopancreas extracts and T-2 also significantly induced IL-6 mRNA gene expression. With reference to phosphorylation levels, significant activation of JAK1 and STAT2 occurred with T-2 and JAK3 by muscle extract, JAK2 by hepatopancreas extract and STAT1 by T-2-GluA. This study showed that both T-2 and mT-2s are cytotoxic but the activation of the JAK/STAT pathway in RAW264.7 cells by T-2 was greater than that by mT-2s in hepatopancreas and muscle extracts from T-2-fed Litopenaeus vannamei.
作者：Wang, X；Wang, YL；Qiu, M；Sun, LJ；Wang, XB；Li, CH；Xu, DF；Gooneratne, R
作者单位：Guangdong Ocean Univ
期刊名称：TOXICOLOGY RESEARCH
期刊影响因子：2.161
出版年份：2017
出版刊次：2
点击下载：T-2和改性T-2毒素的细胞毒性：用T-2毒素喂养的虾的肝胰脏和肌肉提取物诱导RAW264.7细胞中的JAK / STAT通路