中文摘要：黄曲霉毒素B1（AFB1）是由丝状真菌产生的致癌化合物。为了减少食品中AFB1的产生，在体外测试了13株丝状放线菌降低AFB1含量的功效；所有放线菌都是从阿尔及利亚的撒哈拉土壤中分离出来的。研究结果表明这些放线菌菌株可以用于净化处理，减少AFB1的含量。
外文摘要：Aflatoxin B1 (AFB1) is a carcinogenic compound produced by filamentous fungi. In order to reduce AFB1 occurrence in foodstuffs, 13 strains of mycelial actinobacteria were tested in vitro for the efficacy to reduce AFB1 content; all were isolated from the Saharan soils of Algeria. Firstly, morphological study and molecular analysis, based on the 16S rRNA gene, indicated that these strains belong to Actinomadura, Nocardiopsis, Nonomuraea, Saccharothrix and Streptomyces genera. Secondly, each strain’s efficacy to reduce pure AFB1 content was studied in ISP2-medium. After a 4-day incubation at 30°C on AFB1-supplemented medium (5 ppm of AFB1), AFB1 was extracted and quantified. AFB1 content was reduced by all strains (42.9–97.6%). The three most efficient reducers (94.9–97.6%) were two strains belonging to the genus Streptomyces and one to the genus Saccharothrix. Among the latter, strains ACD6 and ABH19 showed no adsorption mechanism involved, suggesting a potential degradation mechanism. These findings led us to suggest that these actinobacterial strains could be used as decontamination treatments for the reduction of AFB1 content.
外文关键词：Mycelial actinobacteria；Taxonomy；Molecular identification；Aflatoxin B1 decontamination
作者：Lahoum, A；Verheecke-Vaessen, C；Bouras, N；Sabaou, N；Mathieu, F
作者单位：Ecole Normale Super
期刊名称：ANNALS OF MICROBIOLOGY
期刊影响因子：1.232
出版年份：2017
出版刊次：3
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