中文摘要：具有支链淀粉增加、天冬酰胺水平降低和黑斑病发病率降低等改良特性转基因马铃薯品种已被开发。本文用用多重聚合酶链反应对韩国未经批准转基因马铃薯（EH92-527-1、AM04-1020、PH05-026-0048和E12）进行检测研究。作者用UDP-葡萄糖焦磷酸化酶基因作为内部参照基因，并用转基因马铃薯和其他非转基因作物进行了引物的特异性分析。多重PCR检测限被确定约为0.04%（w/w）。作者用多重PCR技术对含有马铃薯的33个商业产品进行了检测，并检测到了转基因马铃薯品种E12。研究结果表明，本文所建立的多重PCR技术可以有效地鉴别韩国未经批准的转基因马铃薯。
外文摘要：Several genetically modified (GM) potato cultivars with improved traits such as increased amylopectin levels, decreased asparagine levels, and reduced incidence of black spot have been developed. In this study, we describe a multiplex polymerase chain reaction (PCR) method specific for four GM potato events (EH92-527-1, AM04-1020, PH05-026-0048, and E12) that are unauthorized in Korea. The UDP glucose pyrophosphorylase (UGPase) gene was used as the endogenous reference gene. The specificity of the primer sets was evaluated using GM potatoes and other GM crops. The limit of detection in the developed multiplex PCR was confirmed to be approximately 0.04% (w/w). Thirty-three commercial products containing potato ingredients were tested by the multiplex PCR assay, and GM potato event E12 was found in one of the 33 samples. This result suggested that the developed PCR method could be used to effectively identify unauthorized GM potatoes in Korea.
外文关键词：Genetically modified potato; Polymerase chain reaction; Multiplex polymerase chain reaction; Monitoring
作者：Song, Ji-Yeon; Kim, Jae-Hwan; Kim, Hae-Yeong
作者单位：Kyung Hee Univ
期刊名称：FOOD CONTROL
期刊影响因子：3.496
出版年份：2017
出版刊次：10
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