中文摘要：了解转Bt基因水稻对土壤生态系统的影响是转基因作物环境风险评价的必要条件。虽然转BT基因水稻的短期栽培对土壤性质的影响已有报道，但转BT基因水稻的长期栽培对土壤生态系统影响的研究却不多。本文的土壤样品取自于已连续8年种植转Bt基因水稻（Kefeng-6）及其非转基因亲本品种（minghui-86）的中国福建。作者通过对土壤BT蛋白质含量和16个变量的分析来评价转BT基因水稻栽培对土壤健康造成的潜在风险。研究发现，在转BT基因水稻栽培后，对土壤BT蛋白质不太可能积累，且土壤酶活性（过氧化氢酶、脱氢酶、观察酸性磷酸酶、脲酶）、微生物生物量（微生物碳、氮）、总有机碳、分解作用（土壤呼吸、Q(10)和qCO(2)）、土壤中氮磷含量等均没有发生显著改变。由于当地的传统，收割后地上生物量被去除，因而转BT基因水稻栽培提高净初级生产力但并没有显著改变土壤碳循环。研究结果表明，在土壤微生物功能和碳循环方面，转BT基因水稻的长期栽培不可能对土壤健康产生显著影响。
外文摘要：Understanding how soil ecosystem responds to transgenic Bacillus thuringiensis (Bt) rice is necessary for environmental risk assessment. While the influences of short-term cultivation of Bt rice on soil properties have been reported previously, little is known about the long-term effects of Bt rice on soil ecosystems. In this study, soil samples were taken from a long-term rice cultivation site in Fujian Province, China, where transgenic Bt rice (Kefeng-6) and its non-Bt parent breed (Minghui-86) had been continuously cultivated for 8 years. Soil Bt protein concentration and a total of 16 variables were analyzed to assess potential risks of soil health under Bt rice cultivation. The results revealed that soil Bt protein is unlikely to accumulate after Bt rice cultivated in the field, and no consistently significant changes were observed in soil enzymatic activities (catalase, dehydrogenase, acid phosphatase, and urease), microbial biomass (microbial carbon and nitrogen), total organic carbon, decomposition (soil respiration, Q(10), and qCO(2)), soil nitrogen and phosphorus contents. Due to a local tradition that aboveground biomass was removed after harvest, the increased net primary productivity by Bt rice cultivation did not significantly change soil C cycling. Results of this study suggested that on the aspects of soil microbial functioning and C cycling, long-term cultivation of Bt rice is unlikely to result in significant effects on soil health.
作者：Lee Zhaolei; Bu Naishun; Cui Jun; 等
作者单位：Fudan Univ
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.259
出版年份：2017
出版刊次：7
点击下载：转Bt基因水稻（Kefeng-6）的长期栽培对土壤微生物功能和碳循环的影响