中文摘要：检测食品中的黄曲霉毒素需要及时，以免受污染食品的分配和消费。本研究基于适体（Apt）-适配体（CSs）复合物互补链，其在电极和外切核酸酶I（Exo I）的表面形成PI形结构，提出了一种精确的检测AFB1的电化学传感策略。
外文摘要：There is a prompt need for determination of aflatoxin B-1 (AFB(1)) in food products to avoid distribution and consumption of contaminated food products. In this study, an accurate electrochemical sensing strategy was presented for detection of AFB1 based on aptamer (Apt)-complementary strands of aptamer (CSs) complex which forms a pi-shape structure on the surface of electrode and exonuclease I (Exo I). The presence of n-shape structure as a double-layer physical barrier allowed detection of AFB(1) with high sensitivity. In the absence of AFB(1), the pi-shape structure remained intact, so only a weak peak current was recorded. Upon the addition of AFB(1), the pi-shape structure was disassembled and a strong current was recorded following the addition of Exo I. Under optimal conditions, the electrochemical signals enhanced as AFB(1) concentrations increased with a dynamic range of 7-500 pg/mL and a limit of detection (LOD) of 2 pg/mL. The developed aptasensor was also used to analyze AFB(1) spiked human serum and grape juice samples and the recoveries were 95.4-108.1%.
外文关键词：Aflatoxin B-1；Electrochemical aptasensor；pi-shape；Real samples；Exonuclease I
作者：Abnous,K；Danesh,NM；Alibolandi,M；Ramezani,M；Emrani,AS；Zolfaghari,R；Taghdisi,SM
作者单位：Mashhad Univ Med Sci
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.476
出版年份：2017
出版刊次：94
点击下载：一种用于超灵敏检测黄曲霉毒素B1的新型增强PI形电化学适体传感器