中文摘要：本研究开发了一种基于Ru（bpy）32+掺杂二氧化硅纳米粒子(Ru@SiO2 NPs)的新型分子印迹电化学发光(MIP-ECL)传感器，用于高灵敏检测伏马菌素B1（FB1）。结果表明，本研究成功研制出了一种简易的MIP-ECL传感器，它对FB1的检测限为0.35pg/mL，线性范围从0.001到100ng/mL。而且，该MIP-ECL传感器在实际样品中显示了极好的应用。
外文摘要：A novel molecularly imprinted electrochemiluminescence (MIP-ECL) sensor based on Ru(bpy) 3 2+ -doped silica nanoparticles (Ru@SiO 2 NPs) is developed for highly sensitive detection of fumonisin B 1 (FB 1 ). Gold-nanoparticles (AuNPs), Ru@SiO 2 NPs with chitosan (CS) composites and a molecularly imprinted polymer (MIP) are assembled on a glassy carbon electrode (GCE) to fabricate an ECL platform step by step. AuNPs could greatly promote the ECL intensity and improve the analytical sensitivity according to the localized surface plasmon resonance (LSPR) and the electrochemical effect. In this surface-enhanced electrochemiluminescence (SEECL) system, AuNPs work as the LSPR source to improve the ECL intensity and Ru@SiO 2 NPs are used as ECL luminophores. In the phosphate buffer solution (PBS), the evident anodic ECL of Ru@SiO 2 on the above working electrode is observed in the presence of the template molecule fumonisin B 1 (FB 1 ), which could act as the coreactant of Ru@SiO 2 NPs due to the amino group of FB 1 . When the template molecules were eluted from the MIP, little coreactant was left, resulting in an apparent decrease of ECL signal. After the MIP-ECL sensor was incubated in FB 1 solution, the template molecules rebound to the MIP surface, leading to the enhancement of ECL signal again. On the basis of these results, a facile MIP-ECL sensor has been successfully fabricated, which exhibited a linear range from 0.001 to 100 ng mL(-1) with a detection limit of 0.35 pg mL(-1) for FB1. Moreover, the proposed MIP-ECL sensor displayed an excellent application in real samples.
外文关键词：Electrochemiluminescence；Ru(bpy)(3)(2+)-doped silica nanoparticle；Fumonisin B-1；Gold-nanoparticles；Molecularly imprinted polymer
作者：Zhang, W；Xiong, HW；Chen, MM；Zhang, XH；Wang, SF
作者单位：Hubei Univ
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.476
出版年份：2017
出版刊次：10
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