中文摘要：从伊朗不同地区共收集了360种传统奶酪，包括Lighvan(n=62)、Koozeh(n=62)、Siahmazgi(n=58)、Khiki(n=58)、Talesh(n=58)和Lactic(n =62)，用酶联免疫吸附试验(ELISA)分析了黄曲霉毒素M1(AFM1),并用高效液相色谱荧光检测法(HPLC-FD)检测了所有ELISA阳性样品中AFM1的频率及其浓度范围。研究表明消费伊朗传统奶酪不存在AFM1带来的健康风险。
外文摘要：A total of 360 traditional cheeses consisted of Lighvan (n = 62), Koozeh (n = 62), Siahmazgi (n = 58), Khiki (n = 58), Talesh (n = 58) and Lactic (n = 62) collected from different parts of Iran were analyzed for aflatoxin M-1 (AFM(1)) using an enzyme linked immunosorbent assay (ELISA). Frequency of AFM(1) and its concentration ranges of all the ELISA positive samples were determined by high-performance liquid chromatography with fluorescence detection (HPLC-FD). AFM(1) was detected in 60.3%, 75.8%, 72.4%, 43.5%, 38.7% and 35.4% of Siahmazgi, Khiki, Talesh, Lighvan, Koozeh and Lactic cheeses, respectively with concentration ranging from 50.5 to 308.7 ng/kg, respectively. HPLC analyses confirmed the ELISA results although the rates of contaminated cheese samples were lower than that of ELISA. There was significant difference in AFM1 level between various cheese types and samples collected from summer and winter seasons (P < 0.05). By comparing our findings with the EU limit, about 10.5% of cheese samples had exceeding values for the toxin. The results of the present study indicates that there is no health risk in consumption of Iranian traditional cheeses due to the presence of AFM(1).
外文关键词：Seasonal variation；Risk assessment；Aflatoxin M-1；Traditional cheese；Iran
作者：Shahbazi, Y；Nikousefat, Z；Karami, N
作者单位：Razi Univ
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：9
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